Imaging and Microscopy

The EML offers investigators access to unique expertise and EM technologies that allow CCR Investigators to explore new avenues of research in order to enhance the knowledge of biological systems. To assist our customers, we provide complete specimen preparation service which includes shipment of fixative, processing, embedding, sectioning, and imaging of samples. If desired, investigators can also discuss the design of custom protocols tailored to their specific needs. EML is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National… [learn more]

OMAL focuses its research and development activities to quantitatively understand the molecular basis of three-dimensional (3D) cell organization, motility, invasion, and differentiation using fixed samples and live, 3D tissue culture models (i.e., translational models). OMAL is a CCR-dedicated facility. Prospective users and collaborators should first consult the following website before contacting OMAL staff: OMAL Website. OMAL is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National Laboratory. Established Optical Microsco… [learn more]

The Spatial Imaging Technology Resource (formerly the Nanoscale Protein Analysis Section of the Collaborative Protein Technology Resource or CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery and translation/clinical studies. Applications include: Quantitative profiling of cell signaling and post-translational modification, Measurement of cytokine, metabolite, and serum/plasma markers, Identification and validation of biomarkers & therapeutic targets, Assessment of on‐ … [learn more]

CCR VOLUME Electron Microscopy (CVEM) aims to develop new techniques in volume electron microscopy (vEM) and, in collaboration with CCR Investigators, apply these techniques to research cell biological questions well-suited for study by high-resolution 3D electron microscopy. CVEM, headed by Kedar Narayan, uses focused ion beam scanning electron microscopy (FIB-SEM), array tomography (AT), electron tomography (ET) and other complementary methods to visualize ultrastructural features of cells and tissues, including cell membranes, cellular organelles, and virus par… [learn more]

The Chemistry and Synthesis Center (CSC) of the National Heart, Lung, and Blood Institute (NHLBI) provides IRP scientists with targeted imaging probes and chemical tools that help accelerate cell-based assays, in vivo imaging studies, and translational research leading to better disease diagnosis and therapy. It comprises a core synthesis facility dedicated to the preparation of imaging probes, including radiolabeling for PET and SPECT applications, and a research component dedicated to the discovery of new imaging approaches and compositions. Established Tec… [learn more]

EIB Microscopy Facility has the following instruments: Abberior STEDYCON super-resolution (STED) microscope, Zeiss LSM 880-NLO confocal / two-photon system, Yokogawa CSU-W1 spinning disk confocal microscope, Zeiss AxioObserver Z1 wide-field microscope, As a multi-user facility, the different instruments provide a wide range of imaging modes for EIB scientists, from standard immunohistochemistry, through brightfield and wide-field epifluorescence imaging, to highly complex live cell confocal micro… [learn more]

The LCBG Microscopy Core offers imaging technologies and training. The Core has established instrumentation for for 2D and 3D imaging of both fixed and living specimens.  [learn more]

LCMB Microscopy Core offers live cell imaging technologies as well as super-resolution, fluorescence lifetime and confocal imaging systems for immunofluorescence. Our confocal instruments are a Leica SP8 laser scanning confocal microscope and a Nikon spinning disk confocal microscope. We also house a Total Internal Reflection Fluorescence (TIRF) microscope with capability to perform super-resolved Single Molecule Localization Microscopy and incuabtion chamber equipped epi-fluorescence imaging systems for long-term live-cell imaging experiments. Established Technologies: Live … [learn more]

The core provides access to several different state-of-the-art 3D microscopes as well as computers to visualize and process image data. The facility houses equipment for 2D or 3D imaging of fixed and living specimens. High resolution images can be obtained by confocal microscopy or deconvolution, and super-resolution techniques SIM, STORM, MINFLUX. Single Molecule tracking is supported on MINFLUX, and custom HILO-illumination microscopes. Established Technologies: Facility personnel are available for consultation about the design of imaging experiments and/or the analysis of image da… [learn more]

The NCI High-Throughput Imaging Facility (HiTIF) works in a collaborative fashion with NCI/NIH Investigators by providing them with the necessary expertise, instrumentation, and software to develop and execute advanced High-Throughput Imaging (HTI) assays. These can be paired to screen libraries of RNAi or CRISPR/Cas9 reagents to discover and characterize novel cellular pathways by functional genomics. In addition to functional genomics screens, HiTIF also develops HTI assays to extract and quantify single-cell information about the molecular mechanisms underlying rare or heterogeneous b… [learn more]

The function of the SAIP is to collaborate with NCI investigators in the development of mouse models, new molecular imaging probes for early detection and therapy, monitor tumors in vivo, and perform drug efficacy studies utilizing in vivo imaging techniques. In addition, the SAIP collaborates with the NCI Division of Cancer Treatment and Diagnosis (DCTD) Nanotechnology Characterization Laboratory (NCL) to analyze nanoplatforms as part of the cascade assay and assist DCTD initiatives in developing standards in small animal imaging, integrate imaging into drug development, and develop par… [learn more]

The NHLBI Murine Phenotyping Core carries out physiologic and behavioral testing in a diversity of mouse models for NHLBI and other NIH institutes. Established Technologies: Cardiovascular Phenotyping, Metabolic Phenotyping, Pulmonary Phenotyping, Behavioral Phenotyping, Exercise Physiology, Echocardiography, In vivo functional vascular phenotyping, Advanced Imaging Modalities (whole body high frequency ultrasound imaging, vascular imaging), [learn more]

The facilities at AIM are available for use by the entire NIH intramural research community. While we welcome users with any size imaging project, AIM specializes in large, yearlong (or longer), collaborative research efforts with NIH labs – providing sample preparation, microscope operation and development, and data processing/analysis as needed. We take care of the imaging and processing so that you can focus on the biology. Established Technologies: Microscope Description Spatial Res… [learn more]

The Biomedical Engineering and Physical Science (BEPS) shared resource supports NIH’s intramural basic and clinical scientists on applications of engineering, physics, imaging, measurement, and analysis. BEPS is centrally located on the main NIH campus and provides expertise that spans technologies ranging in scale from near-atomic resolution to intact organisms. Established Technologies: Electron Microscopy The Electron Microscopy Unit provides instrumentation, training, and services for: immuno electron microscopy, electron tomography, and specimen preparation, including cryo-techniq… [learn more]

The Mouse Imaging Facility (MIF) is a shared, trans-NIH intramural resource for animal imaging studies. MIF provides access to state-of-the-art radiological imaging methods optimized for mice, rats, other animals, and tissue samples. MIF provides intellectual, technical, and material support for investigators using the MIF imaging facilities. The MIF is supported by all institutes at the NIH and reports to the Shared Resources Subcommittee (SRS) of the Scientific Directors. Administratively, the MIF resides within NINDS and is physically located in the NIH MRI Research Center (NMRC) … [learn more]

The NIH MRI Research Facility (NMRF) is a shared, intramural resource for human imaging studies. The NMRF is supported by all Institutes at NIH and reports to the Shared Resources Subcommittee (SRS) of the Scientific Directors. Administratively, NMRF resides within NINDS. The NMR Center Steering Committee provides the direct oversight for NMRF activities. Service Offerings Provide intellectual, technical and material support for investigators using its imaging and computer resources. , MRI using a state-of-the-art 7.0 T human MRI system and other resour… [learn more]

The Pathology Service, Division of Veterinary Resources (DVR), Office of Research Services (ORS), provides diagnostic gross and histopathologic evaluation of any species of laboratory animals used in the NIH Intramural Research Program due to morbidity, mortality, or unexpected findings. In addition, mouse phenotyping and transmission electron microscopy services are available. Established Technologies The Pathology Service offers a Mouse Phenotyping Service with comprehensive pathologic analysis for genetically engineered mice. The Service provides standardized gro… [learn more]

The CCR Microscopy Core provides NCI investigators access to state-of-the-art imaging tools and techniques, including light sheet fluorescence, high-resolution confocal, multi-photon, and super-resolution microscopy. The mission of the CCR Microscopy Core Facility is to support the microscopy and digital imaging needs of investigators studying the biological structures and cellular processes involved in the cell biology of cancer by applying our diverse imaging resources, expertise, and quality customer service. The goal is to provide cutting-edge imaging technolo… [learn more]

The FNLCR Molecular Histopathology Laboratory (MHL) provides comprehensive veterinary pathology support for animal health monitoring, biomarker discovery and validation, drug development, genomics, and proteomics on a cost recovered basis. The MHL is organized into multiple process groups with cross-trained experts in animal study design, phenotyping of genetically engineered mice (GEM), immunopathology, anatomic pathology, toxicological pathology, and specimen classification/selection for tissue microarray (TMA) construction or laser capture microdissection (LCM). Established… [learn more]