Bethesda, MD
Trans NIH Facility
The National Institutes of Health (NIH) Office of Research Services (ORS) Division of Radiation Safety (DRS) Radioactive Materials Control and Analysis Branch supports radioactive materials packaging/shipping. Established Technologies In accordance with Department of Transportation ( Read More...
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Bioinformatics
The goal of quality control is to keep only high quality cells (i.e., remove low quality cells (dead or dying cells), cell-free RNA, or doublets). Low quality cells will impact downstream analyses. Take care Read More...
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Bioinformatics
01/30/2024 - This class introduces version control and how to use GitHub for project versioning. Participants will have a better understanding of version control, GitHub, and their advantages for managing projects. Upon Read More...
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Bioinformatics
SCAF: Overview of Cell Ranger output files and single cell data analysis quality control Kimia Dadkhah, bioinformatics analyst (SCAF), will talk about Cell Ranger output and essential quality control metrics in single cell data analysis, Read More...
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Bioinformatics
Kimia Dadkhah, bioinformatics analyst (SCAF), will talk about Cell Ranger output and essential quality control metrics in single cell data analysis, how to interpret these and make informed decisions, and other considerations to keep in Read More...
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Bioinformatics
04/17/2024 - Kimia Dadkhah, bioinformatics analyst (SCAF), will talk about Cell Ranger output and essential quality control metrics in single cell data analysis, how to interpret these and make informed decisions, and other considerations to keep Read More...
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Bioinformatics
02/21/2024 - Versioning enables researchers to track changes in coding projects. This Coding Club session will introduce the versioning tool GitHub (https://github.com). At the end of this class, participants will Become familiar with options Read More...
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Bioinformatics
01/21/2025 - About the Speaker: Hari completed his PhD in Cell Biology at the University of Toronto, where he used single-molecule imaging techniques to reveal how multimolecular interactions govern early signaling events in individual immune cells. Read More...
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Bioinformatics
05/23/2024 - Versioning enables researchers to track changes in coding projects. This Coding Club introduces Git (https://git-scm.com), an open-source software used to perform versioning on a personal computer. At the end of this class, Read More...
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Bioinformatics
02/28/2024 - Versioning enables researchers to track changes in coding projects. This Coding Club session will introduce Git (https://git-scm.com), an open-source software used to perform versioning locally and enables users to upload code to Read More...
Frederick, MD
Collaborative
The Biopharmaceutical Development Program (BDP) provides resources for the development of investigational biological agents. The BDP supports feasibility through development and Phase I/II cGMP manufacturing plus regulatory documentation.The BDP was established in 1993. We Read More...
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Bioinformatics
06/24/2020 - Join us for a webinar: How to Analyze Single Cell RNA-Seq Data: Point, Click, Done Register: https://www.partek.com/webinar/how-to-analyze-single-cell-rna-seq-data-point-click-done/ Single cell mRNA sequencing allows for the identification of different cell subtypes Read More...
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Bioinformatics
02/20/2025 - This class will introduce the first step in analyzing NGS data using bulk RNA sequencing as an example. After attending, participants will be able to describe the file format in which NGS data is Read More...
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Bioinformatics
01/24/2024 - Dear colleagues, Please join us on Wed., Jan. 24 when Dr. Avi Ma’ayan from the Icahn School of Medicine at Mount Sinai will demonstrate how to use these tools to access hundreds of thousands Read More...
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Bioinformatics
Changes to a coding project (including scripts, data, and other content) should be saved periodically, similar to clicking on the "save" button to periodically save changes when constructing a word document. This is Read More...
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Bioinformatics
04/24/2025 - In this lesson, attendees will learn the most basic features of the R programming language. The focus will be on R syntax, R objects, and data types.
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Bioinformatics
When using SingleR, the 3 primary parameters are the experimental dataset, the reference dataset, and the labels being used. Continuing with the main labels of the MouseRNASeq dataset on the full dataset looks like this: annot = Read More...
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Bioinformatics
Welcome to Getting Started with scRNA-Seq This is a mini seminar series designed to help attendees learn more about single cell RNA-Seq, from applicable technologies to data analysis. Seminar Schedule April 3, 2024 - The CCR Single Read More...
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Bioinformatics
All seminars will be recorded and made available on the BTEP Video Archive 24 to 48 hours following the event.
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Bioinformatics
April 3, 2024 - The CCR Single Cell Analysis Facility (SCAF): An Overview (Mike Kelly, SCAF) ( Recording ) April 10, 2024 - Introduction to single cell RNA-Seq (Charlie Seibert, Saeed Yadranji Aghdam, SCAF) ( Recording ) April 17, 2024 - SCAF: Overview of Cell Read More...
Bethesda, MD
Core Facility
The core provides access to several different state-of-the-art 3D microscopes as well as computers to visualize and process image data. The facility houses equipment for 2D or 3D imaging of fixed and living specimens. High Read More...
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Bioinformatics
06/24/2025 - In thi le on, we will learn how to tidy me y data u ing function from the tidyver e package, tidyr . The primary focu will be on re haping data from wide to Read More...
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Bioinformatics
06/24/2025 - Thi year the Frederick Re earch Compute Environment (FRCE) i expanding it outreach. Our fir t new feature i a web-ba ed tool that will allow you to ea ily run graphical application on Read More...
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Bioinformatics
04/24/2025 - This class will introduce beginners or those looking for a refresher to Jupyter Lab, a platform used to organize code and analysis steps in one place. Jupyter Lab can be easily installed or run Read More...
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Bioinformatics
03/24/2025 - AI Club is a weekly meeting that explores various topics relating to AI and deep learning in biomedical sciences, typically in a seminar, workshop, or journal club format. AI Club is intended to be Read More...
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Bioinformatics
02/24/2025 - AI Club is a weekly meeting that explores various topics relating to AI and deep learning in biomedical sciences, typically in a seminar, workshop, or journal club format. AI Club is intended to be Read More...
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Bioinformatics
10/10/2024 - This introductory lecture will provide an overview of bulk RNA-Seq technology, its various application and shortcomings, as well as detailing the steps involved in analyzing the resulting data. The talk is aimed at those Read More...
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Bioinformatics
04/24/2024 - This seminar provides an introduction to R in the context of single cell RNA-Seq analysis with Seurat. In this seminar, attendees will learn about options for analyzing scRNA-Seq data, resources for learning R, how Read More...
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Bioinformatics
04/24/2024 - Please join us on Wednesday, April 24, 2024, when Dr. Abhishek Jha, co-founder and CEO of Elucidata, will present " Data Quality for LLMs: Building a Reliable Data Foundation." The presentation starts at 11:00 a.m. Read More...
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Bioinformatics
Now we are ready to work with some of our first R commands. We are going to run commands directly from our R script rather than typing into the R console. Our first command will Read More...
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Bioinformatics
To participate in this class you will need your government-issued computer and a reliable internet connection. You do not need to download or install any software to participate in the class. However, at the end Read More...
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Bioinformatics
01/24/2024 - This 90-minute course equips participants with essential knowledge and skills for effective interactions with Large Language Models (LLMs), such as ChatGPT. Explore the intricacies of prompt engineering and its pivotal role in optimizing the Read More...
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Bioinformatics
01/24/2024 - Documenting your data analysis is a crucial step toward making your research reproducible. In this session of the BTEP Coding Club, we will learn how to get started using Quarto with RStudio for report Read More...
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Bioinformatics
01/24/2024 - Dear Colleague, Helen Shearman, Ph.D., Senior Field Application Scientist, will be presenting a one-hour overview demonstration on SnapGene. SnapGene offers a fast and easy way to plan, visualize, and document your Read More...
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Bioinformatics
To create an R object, you need a name, a value, and an assignment operator (e.g.,
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Back Services: Biophysics Facility offers MDS as an open-access instrument. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Training includes the KD determination of a standard molecular Read More...
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Back Services: Biophysics Facility offers MST as an open-access instrument. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Training includes the KD determination of a Read More...
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CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Research Festival The NIH Research Festival highlights the groundbreaking science and the vibrant NIH community driving our Read More...
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CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New NIH Resource Spotlight The NIH Lab Managers Working Group have developed a new NIH-wide database of cold Read More...
Frederick, Maryland
Collaborative
Bruker AVANCE 400 and 500 MHz NMR instruments. Helium Cryoprobe technology on the 500 MHz machine for added sensitivity, especially for Carbon-13 spectra. Access to a second 500 MHz instrument with Prodigy Liquid Nitrogen-cooled cryoprobe. User Accounts can be Read More...
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Bioinformatics
07/24/2025 - NIDDK Bio tat eminar erie : From Re earch tudy De ign to Collecting, Managing, and Analyzing Data. Learning Objective : 1. To delineate feature of REDCap to upport project management for re earch tudie . 2. To outline Read More...
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Bioinformatics
07/24/2025 - The "Data Vi ualization in R" erie focu e on u ing ggplot and the broader tidyver e eco y tem to create in ightful and cu tomizable vi ualization . It cover Read More...
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Bioinformatics
06/24/2025 - Tran late gene li t into biological in ight u ing pathway enrichment tool . Thi training will provide an overview the current tatu of pathway tool , with focu on oftware available to NIH community. Read More...
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Bioinformatics
06/17/2025 - Explore tran cription factor binding ite analy i and peak calling. Thi training will introduce ChIP- eq data analy i followed by a tep-by- tep live demon tration of a ChIP- eq analy i Read More...
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Bioinformatics
06/10/2025 - Under tand how to identify and interpret variant . Thi training will introduce Exome equencing data analy i followed by a tep-by- tep live demon tration of an exome equencing analy i workflow u ing Read More...
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Bioinformatics
01/22/2025 - Alzheimer’s Disease (AD) presents significant challenges in prevention and treatment despite decades of research advancements. Innovative AI/ML approaches enable analysis of real-world data sources, such as electronic health records (EHRs) Read More...
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Bioinformatics
12/05/2024 - This one and a half hour online training will provide a demonstration of how to identify cell types based on statistics, visualization, and canonical markers. One Peripheral blood mononuclear cells (PBMCs) sample will be Read More...
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Bioinformatics
12/03/2024 - This one and a half hour online training will provide a demonstration of how to build a Bulk RNA-Seq data analysis pipeline using a fastq file. Partek Flow Read More...
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Bioinformatics
What is bioinformatics? Bioinformatics integrates biology, statistics, and computer science to develop and apply theory, methods, and tools for the collection, storage, and analysis of biological and related data. Bioinformatics plays a critical role in Read More...
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Bioinformatics
11/15/2024 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...
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Bioinformatics
11/07/2024 - Dr. Blackshaw's work investigates the molecular mechanisms controlling neurogenesis and cell fate specification in the vertebrate forebrain, with a particular focus on the retina. He currently focuses on the use of comparative S Read More...
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Bioinformatics
10/24/2024 - Recent advances in artificial intelligence (AI) have revolutionized the use of hematoxylin and eosin (H&E)-stained tumor slides for precision oncology, enabling data-driven approaches to predict molecular characteristics and therapeutic outcomes. In Read More...
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Bioinformatics
09/24/2024 - This class will introduce beginners or those looking for a refresher to Jupyter Lab, a platform used to organize code and analysis steps in one place. Jupyter Lab can be easily installed or run Read More...
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Bioinformatics
07/24/2024 - As a cancer researcher, did you know that data sharing is now required by many funding agencies and journals? Join NCI CBIIT’s Dr. Jill Barnholtz-Sloan & Read More...
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Bioinformatics
07/24/2024 - Knowledge of Unix command line is advantageous for scientists who are new to bioinformatics, as many tools are designed to run on Unix-like systems. High performance computing systems (e.g., NIH Biowulf) also require Read More...
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Bioinformatics
07/24/2024 - Partek Flow enables scientists to construct analysis workflows for multi-omics sequencing data including DNA, bulk and single cell RNA, spatial transcriptomics, ATAC and ChIP. It is hosted on Biowulf, the NIH high performance computing Read More...
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Bioinformatics
07/24/2024 - This hour and half in-person training will explore the topics of perception and cognition, and how these apply to data visualization. There will also be a discussion on “pre-attentive” properties Read More...
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Bioinformatics
07/09/2024 - Galaxy is a scientific workflow, data integration, data analysis, and publishing platform that makes computational biology accessible to research scientists that do not have computer programming experience. This workshop will cover exome sequencing data Read More...
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Bioinformatics
06/04/2024 - Galaxy is a scientific workflow, data integration, data analysis, and publishing platform that makes computational biology accessible to research scientists that do not have computer programming experience. This training will introduce ChIP sequencing data Read More...
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Bioinformatics
The quality metric information is stored in the metadata ( adp@meta.data ), so we really only need to work with the metadata file to get an idea of the thresholds we want to set for Read More...
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Bioinformatics
Much like pseudobulk differential expression, the RNA expression can be collapsed into pre-defined components, such as the clusters, if it is believed that cell-to-cell variation is inducing too much confusion in the labeling. This collapsing Read More...
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Bioinformatics
Now, we can either use our filtering parameters directly with subset() or provide a cells argument. # use different parameters; established above adp_filt 350 & nCount_RNA >650 & percent.mt
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Bioinformatics
This tutorial was designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples. Throughout this tutorial we will Apply Read More...
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Bioinformatics
05/13/2024 - This in-person workshop will focus on data wrangling using tidy data principles. Tidy data describes a standard way of storing data that facilitates analysis and visualization within the tidyverse ecosystem. There will be a Read More...
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Bioinformatics
04/21/2024 - Looking for an introduction to artificial intelligence (AI) or an opportunity to expand on what you already know? Attend this workshop hosted by the NIH-funded Bridge2AI CHoRUS network and the University of Florida Read More...
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Bioinformatics
03/05/2024 - This in-person workshop will show participants how to identify cell types based on statistics, visualization, and canonical markers. One Peripheral blood mononuclear cells (PBMCs) sample will be used to illustrate a basic Single Cell Read More...
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Bioinformatics
There is an approach to data analysis known as "split-apply-combine", in which the data is split into smaller components, some type of analysis is applied to each component, and the results are combined. Read More...
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Bioinformatics
Once you have intiated your document, you can get started documenting your analysis. There are three basic components to a quarto document: yaml header (bracketed by --- ) The yaml header or file allows us to Read More...
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Bioinformatics
There is an approach to data analysis known as "split-apply-combine", in which the data is split into smaller components, some type of analysis is applied to each component, and the results are combined. Read More...
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Bioinformatics
Reproducibility in science means being able to generate the same experimental / analytical results with a high degree of reliability. This is necessary for research validation, scientific and public trust, innovation, and collaboration. Reproducibility is not Read More...
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Bioinformatics
There are data sets available in R to practice with or showcase different packages. For today's lesson and the remainder of this course, we will use data from the Bioconductor package airway to showcase Read More...
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Bioinformatics
Because we are working on DNAnexus, and our files will not remain at the end of each class, we aren't going to use a R project for all lessons. However, it is worth creating Read More...
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Bioinformatics
Course Overview Welcome to the R Introductory Series! A series of introductory lessons in R for scientists. This course will include a series of lessons for individuals new to R or with limited R experience . Read More...
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Bioinformatics
01/24/2024 - This is the second lesson of the Introduction to Unix on Biowulf, January 2024 series. After this lesson, participants will Know how to get help with Unix commands Know how to transfer data from local Read More...
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Bioinformatics
01/23/2024 - This class focuses on using Git and GitHub, with RStudio. Using integrated RStudio tools, participants will have a chance to experiment with this integration and understand its advantages for collaboration and managing projects. You Read More...
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Bioinformatics
For the following plots, let's use the diamonds data ( ?diamonds ). The diamonds dataset comes in ggplot2 and contains information about ~54,000 diamonds, including the price, carat, color, clarity, and cut of each diamond. --- R4 Read More...
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Bioinformatics
There are many steps that can be taken following subsetting (i.e., filtering by rows and columns); one of which is reordering rows. In the tidyverse, reordering rows is largely done by arrange() . Arrange will Read More...
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Bioinformatics
Which of the following will throw an error and why? 4 _ chr :1:2: unexpected input ## 1: 4_ ## ^ . 4 chr :1:3: unexpected symbol ## 1: .4chr ## ^ {{Edet}} Create the following objects; give each object an appropriate name (your best guess at what name to Read More...
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Bioinformatics
This is our first coding help session. We have designed some practice problems to get you acquainted with using R before beginning to wrangle in our next lesson. Practice problems Which of the following will Read More...
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Bioinformatics
In lesson 3, we learned how to read and save excel spreadsheet data to a R object using the tidyverse package readxl . Today we will use some example data from an excel spreadsheet to learn the Read More...
Frederick, MD
Core Facility
Repositories
The Mouse Modeling Core assists NIH investigators by generating and preserving genetically-engineered mouse strains. Services include scientific consultation, gene-targeting in mouse embryonic stem cells, micro-injection of nucleic acids, proteins, or ES cells into mouse embryos, Read More...
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CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New CREx Program Manager The CREx Team is happy to announce Deepika Velampati as the new CREx Program Read More...
Bethesda, MD
Collaborative
The COP evaluates novel therapies in pet dogs with cancer to improve outcomes for human patients and established the Comparative Oncology Trial Consortium (COTC), a collaborative effort of NCI and extramural comparative oncology centers at 24 Read More...
Bethesda, MD
Trans NIH Facility
The Stem Cell Unit (SCU) has established a database to which hESC lines, adult stem cells and induced pluripotent stem (iPS) cells may be directly compared. These data give scientists the information they need when Read More...
Bethesda, MD
Trans NIH Facility
The NIH Clinical Center Pharmacy Department provides pharmaceutical care to inpatients and outpatients on NIH intramural research protocols at the NIH Clinical Center. The Clinical Center facility encompasses 200 inpatient beds, 93 day-hospital stations, and 15 clinics. Clinical Read More...
Frederick, MD
Core Facility
Molecular Cytogenetics Core Facility facilitates the assessment of structural and numerical genomic changes in pre-cancer and cancer research models. This core provides comprehensive support for the cytogenetic analysis of cells from human and research animal Read More...
Bethesda, MD
Core Facility
The CCR Building 41 Flow Cytometry Core is a full-service facility within the Center for Cancer Research that supports over 150 users representing 26 laboratories. The Core Facility provides instrument and software training, technical expertise, assay development, and Read More...
Frederick, Maryland
Core Facility
Repositories
The Biological Products Core provides the AIDS research community with high-quality purified preparations of various strains of Human Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV), economically prepared by leveraging the economy of scale. Materials Read More...
Bethesda, MD
Core Facility
The CCR Genomics Core is located in Building 41 on the NIH Bethesda campus. The primary goal of the Core is to provide investigators from CCR/NCI and other NIH Institutes access to genomic technologies and Read More...
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CREx News & Updates February 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight ORS Division of Veterinary Resources (DVR) DVR is the central NIH lab animal support program Read More...
Frederick, Maryland
Core Facility
CLIA-Certified Technologies Offered: Fragment Analysis for Micro-satellite Instability Detection, Pharmacoscan Array for Pharmacogenomics, Mutation Detection for PCR and Sanger Sequencing, DNA extraction from whole blood, saliva, FFPE tissues, buccal swabs, nails, hair, PBMCs, buffy coats, Read More...
Bethesda, MD
Core Facility
The Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Established Technologies Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), Intracellular markers, including cytokines and Read More...
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CREx News & Updates March 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight NIA Nonhuman Primate Core The NIA Nonhuman Primate Core supports multi-disciplinary translational aging projects for Read More...
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[tabby title="Home"] About NICE-NIH Intramural CryoEM Consortium NIH Intramural CryoEM Consortium (NICE) serves intramural investigators in all NIH IC’s. NICE provides access to state-of-the-art Titan Krios cryo-electron microscopes for atomic-resolution structure determination of Read More...
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Back Services: Biophysics Facility offers fluorometers as open-access instruments. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Location: Building 50, room 3226 Description: Some substances reemit light after Read More...
Frederick, MD
Collaborative
In order to meet increasing demands from both NIH intramural and extramural communities for access to a small angle X-ray scattering (SAXS) resource, the Center for Cancer Research (CCR) under the leadership of Drs. Jeffrey Read More...
Bethesda, MD
Collaborative
Repositories
The National Cancer Institute (NCI) is developing a national repository of Patient-Derived Models (PDMs) comprised of patient-derived xenografts (PDXs), patient-derived organoids (PDOrg), and in vitro patient-derived tumor cell cultures (PDCs) and cancer-associated fibroblasts (CAFs). These Read More...
Bethesda, MD
Trans NIH Facility
The Science and Technology Research Infrastructure for Discovery, Experimentation, and Sustainability (STRIDES) Initiative The STRIDES Initiative aims to help NIH and its institutes, centers, and offices (ICOs) accelerate biomedical research by reducing barriers in utilizing Read More...
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Back Services: We offer a limited sample processing service using standard SEC-MALS and FFF protocols. This service is intended for the occasional users of this system. Researchers who expect to use this instrument Read More...
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Back Services: We offer a limited sample processing service using standard SEC-MALS and FFF protocols. This service is intended for the occasional users of this system. Researchers who expect to use this instrument Read More...
Bethesda, Maryland
Core Facility
Repositories
The AgingResearchBiobank was officially launched in January 2019 with a mission to provide a state-of-the-art inventory system for the storage, maintenance, and distribution of de-identified biospecimens and associated phenotypic, clinical, and imaging data from numerous NIA-funded Read More...
Bethesda, MD
Core Facility
The Biophysics Core’s mission is to provide support in the study of macromolecular interactions, dynamics, and stability by offering consultations, training, professional collaborations, and instrument access. General Services: Multi-technique molecular interaction studies, Kinetic and Read More...
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CREx News & Updates August 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight NCATS Functional Genomics Laboratory (FGL) FGL is designed to help NIH Investigators use the latest Read More...
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Back Services: Biophysics Facility offers Octet as an open-access instrument. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Training includes a full analysis of a Read More...
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CREx News & Updates May 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Click below to learn how easy it is to navigate the CREx platform. These short videos will Read More...
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Services: Biophysics Facility offers CD as an open-access instrument. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Location: Building 50, room 3123 Description: CD spectroscopy measures the difference Read More...
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Back Services: Biophysics Facility offers CD as an open-access instrument. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Location: Building 50, room 3123 Description: CD spectroscopy measures the Read More...
Rockville, MD
Trans NIH Facility
NISC’s role within NHGRI, and more broadly across NIH, aims to advance genome sequencing and its many applications, with a goal not simply to produce sequence data, but to produce the infrastructure required to Read More...
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The OSTR offers cutting-edge technology platforms to the CCR scientific community through centralized facilities. The videos accessed through this page are designed to introduce the various scientific methodologies OSTR makes available through the cores on Read More...
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Bioinformatics
1. Introduction and Learning Objectives This tutorial has been designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples that Read More...
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Bioinformatics
Most BTEP courses include detailed course materials including lesson content, additional resources, and lesson associated data. These course materials are listed here so that learners can easily return to and review concepts taught in class Read More...
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Bioinformatics
04/16/2025 - Updated Location: ATRF, Frederick MD, Main Auditorium What to bring: Laptop capable of connecting to internet via NIH wifi For questions or to register, please contact Amy Stonelake ( amy.stonelake@nih.gov ) Read More...
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Bioinformatics
04/15/2025 - What to bring: Laptop capable of connecting to internet via NIH wifi Are you looking to expand the reach of your sequencing to enable what long read technologies can provide?& Read More...
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Bioinformatics
General questions or comments about the BTEP program or classes should be addressed to: NCIBTEP@nih.gov Desiree Tillo, Ph.D . Staff Scientist desiree.tillo@nih.gov I have a broad research background in bioinformatics Read More...
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Bioinformatics
Listed below are the video recordings of past BTEP events (classes, seminars, workshops). Videos are hosted on various servers and may play slightly differently. Some videos may be downloaded for local viewing. Recorded Videos of Read More...
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Bioinformatics
10/24/2024 - NIH Text Mining and Natural Language Processing SIG is pleased to welcome you to this special event featuring two extraordinary speakers focused on the applications of Deep Learning in Computational Biology. & Read More...
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Bioinformatics
Congratulations, your experiments have been completed and you have a large amount of data. Now, it’s time to analyze these data. But where do you begin? How can you gain the most meaning from Read More...
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Bioinformatics
Some tools have been described in the previous session (see here ). Today, we will be focusing on the SingleR tool, which also requires the celldex package . In short, SingleR operates by comparing your current dataset Read More...
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Bioinformatics
Now that we have loaded the package, we can import our data. Generally, in R programming, functions that involve data import begin with "read / Read". Seurat includes a number of read functions for Read More...
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Bioinformatics
Once we have read in the matrices, the next step is to create a Seurat object. The Seurat object will be used to store the raw count matrices, sample information, and processed data (normalized counts, Read More...
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Bioinformatics
Learning Objectives This tutorial was designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples. Throughout this tutorial we Read More...
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Bioinformatics
This lesson provides an introduction to R in the context of single cell RNA-Seq analysis with Seurat. Learning Objectives Learn about options for analyzing your scRNA-Seq data. Learn about resources for learning R programming. Learn Read More...
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Bioinformatics
03/22/2024 - This 3-hour seminar is tailored for biologists, data analysts, and researchers who are eager to dive into the essentials of computational flow cytometry analysis using R. Flow cytometry is a crucial technique in cell Read More...
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Bioinformatics
Objectives To explore Bioconductor, a repository for R packages related to biological data analysis. To learn about options for report generation with R: RMarkdown and Quarto. Introducing Bioconductor Bioconductor is both an open source project Read More...
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Bioinformatics
Learning Objectives To understand: 1. the difference between R and RStudioIDE. 2. how to work within the RStudio environment including: creating an Rproject and Rscript navigating between directories using functions obtaining help how R can enhance data Read More...
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Bioinformatics
In this lesson, attendees will learn how to transform, summarize, and reshape data using functions from the tidyverse. Learning Objectives Continue to wrangle data using tidyverse functionality. To this end, you should understand: how to Read More...
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Bioinformatics
In this lesson, attendees will learn how to transform, summarize, and reshape data using functions from the tidyverse. Learning Objectives Continue to wrangle data using tidyverse functionality. To this end, you should understand: how to Read More...
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Bioinformatics
Learning Objectives Learn about data structures including factors, lists, data frames, and matrices. Load, explore, and access data in a tabular format (data frames) Learn to write out (export) data from the R environment Data Read More...
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Bioinformatics
Help Session Lesson 4 Plotting with ggplot2 For the following plots, let's use the diamonds data ( ?diamonds ). The diamonds dataset comes in ggplot2 and contains information about ~54,000 diamonds, including the price, carat, color, clarity, and Read More...
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Bioinformatics
Why did we focus so heavily on the tidyverse if it can't be used to manipulate Bioconductor objects? Well, for one, regardless of whether you are a user of Bioconductor packages, you will often Read More...
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Bioinformatics
Let's grab some data. library ( tidyverse ) acount_smeta % dplyr :: rename ( "Feature" = "...1" ) acount #differential expression results dexp % filter ( ! Feature %in% dexp $ feature ) ## # A tibble: 48,176 × 9 ## Feature SRR1039508 SRR1039509 SRR1039512 SRR1039513 SRR1039516 SRR1039517 ## ## 1 Read More...
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Bioinformatics
Help Session Lesson 6 Let's grab some data. library ( tidyverse ) acount_smeta % dplyr :: rename ( "Feature" = "...1" ) acount #differential expression results dexp % filter ( ! Feature %in% dexp $ feature ) ## # A tibble: 48,176 × 9 ## Feature SRR1039508 SRR1039509 SRR1039512 Read More...
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Bioinformatics
In tab delimited files, data columns are separated by tabs. To import tab-delimited files there are several options. There are base R functions such as read.delim() and read.table() as well as the readr Read More...
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Bioinformatics
All solutions should use the pipe. Import the file "./data/filtlowabund_scaledcounts_airways.txt" and save to an object named sc . Create a subset data frame from sc that only includes the columns Read More...
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Bioinformatics
Help Session Lesson 5 All solutions should use the pipe. Import the file "./data/filtlowabund_scaledcounts_airways.txt" and save to an object named sc . Create a subset data frame from sc that only Read More...
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Bioinformatics
R Crash Course: A few things to know before diving into wrangling Learning the Basics Objectives 1. Learn about R objects 3. Learn how to recognize and use R functions 4. Learn about data types and accessors Console Read More...