Search Results for: in vitro RNA synthesis

Introduction to single cell RNA-Seq Single cell RNA sequencing (scRNA-Seq) is becoming increasingly more common in biomedical research, but what is scRNA-Seq? How does it differ from other transcriptomic approaches (e.g., bulk RNA-Seq), and Read More...

Single cell RNA sequencing (scRNA-Seq) is becoming increasingly more common in biomedical research, but what is scRNA-Seq? How does it differ from other transcriptomic approaches (e.g., bulk RNA-Seq), and what are the potential applications, Read More...

05/07/2024 - Galaxy is a scientific workflow, data integration, data analysis, and publishing platform that makes computational biology accessible to research scientists that do not have computer programming experience. This workshop will introduce RNA-seq data analysis Read More...

04/10/2024 - Single cell RNA sequencing (scRNA-Seq) is becoming increasingly more common in biomedical research, but what is scRNA-Seq? How does it differ from other transcriptomic approaches (e.g., bulk RNA-Seq), and what are the potential Read More...

Central Dogma of Molecular Biology What is RNA Sequencing? How to Design an RNA-Seq Experiment Biological and Technical Replicates

Purpose of RNA sequencing and what biological questions can RNA sequencing answer Experimental considerations Sample preparation Replicates Technical noise Read depth More depth for low expression genes More depth for low expression differences between samples Read More...

Introduction to classification base RNA sequencing Review of RNA sequencing

Purpose of RNA sequencing and what biological questions can RNA sequencing answer Experimental considerations Sample preparation Replicates Technical noise Read depth More depth for low expression genes More depth for low fold differences Read length Read More...

Purpose of RNA sequencing and what biological questions can RNA sequencing answer Experimental considerations Sample preparation Replicates Technical noise Read depth More depth for low expression genes More depth for low fold differences Read length Read More...

In this module, we will use the Human Brain Reference and Universal Human Reference RNA sequencing datasets to learn about RNA sequencing. Each lesson will be followed by a practice session where you can ask Read More...

Lessons focus on RNA-Seq analysis including experimental design and best practices, quality control, trimming, alignment based methods, classification based methods, feature counts, and differential expression analysis. Lesson 8: Introduction to RNA-Seq ( Recording ) Lesson 9: Introduction to the Read More...

06/13/2023 - Many cancer-related independent studies that employ bulk and single cell RNA-seq can be found in the Gene Expression Omnibus (GEO). While some studies provide aligned read files, these are processed non-uniformly. This shortcoming makes Read More...

05/12/2023 - Galaxy is a scientific workflow, data integration, data analysis, and publishing platform that makes computational biology accessible to research scientists that do not have computer programming experience. This workshop will introduce RNA-seq data analysis Read More...

01/25/2023 - Bulk RNA-Seq data analysis - learn all about expression counts (raw counts, FPKM, RPKM, TMM, TPM, CPM). Those of you, who are hands-on with RNA-seq, or even simply reading publications on this know there Read More...

11/10/2022 - Participants will learn how to identify cell populations and detect differentially expressed genes in a simple Single Cell RNA-Seq experiment with the point-and-click interface in Partek Flow. By completing this session, attendees will acquire Read More...

11/04/2022 - Participants will learn how to use the point-and-click interface in Partek Flow for RNA-Seq analysis to go from raw data to experimental results. By completing this session, attendees will acquire a working knowledge of Read More...

05/25/2022 - This training will provide an introduction to RNA-seq data analysis followed by tutorials showing the use of popular RNA-seq analysis packages and preparing participants to independently run basic RNA-Seq analysis for expression profiling. The Read More...

01/20/2022 - Participants will learn how to use the point-and-click interface in Partek Flow for RNA-Seq analysis to go from raw data to experimental results. By completing this session, attendees will acquire a working knowledge of Read More...

04/15/2025 - What to bring:  Laptop capable of connecting to internet via NIH  wifi Are you looking to expand the reach of your sequencing to enable what long read technologies can provide?& Read More...

03/21/2025 - Partek Flow is a point-and-click software for analyzing multi-omics sequencing data including DNA, bulk and single cell RNA, spatial transcriptomics, ATAC and ChIP. Input options include FASTQ, BAM, or expression tables, thus enabling scientists Read More...

03/11/2025 - In this class, participants will get hands-on experience with generating and interpreting differential gene expression analysis results from bulk RNA sequencing. In addition, participants will learn to generate common plots used to visualize bulk Read More...

03/04/2025 - Alignment of RNA sequencing data enables researchers to identify where in the genome each sequence came from. However, it does not inform of how many sequences aligned to each genomic feature such as a Read More...

12/05/2024 - This one and a half hour online training will provide a demonstration of how to identify cell types based on statistics, visualization, and canonical markers. One Peripheral blood mononuclear cells (PBMCs) sample will be Read More...

12/03/2024 - This one and a half hour online training  will provide a demonstration of how to build a Bulk RNA-Seq data analysis pipeline using a fastq file. Partek  Flow   Read More...

11/19/2024 - Partek Flow enables scientists to construct analysis workflows for multi-omics sequencing data including DNA, bulk and single cell RNA, spatial transcriptomics, ATAC and ChIP. It is a point-and-click software hosted on Biowulf, the NIH Read More...

06/12/2024 - Join us for an engaging training session where we will examine the similarities and differences between machine learning and statistical differential gene expression (DGE) analysis using Qlucore Omics Explorer. Qlucore Omics Explorer is a Read More...

05/29/2024 - This talk will cover a scRNA-seq workflow available to NCI researchers on NIDAP. NIDAP, the NIH Integrated Data Analysis Platform, is a cloud-based and collaborative data aggregation and analysis platform that hosts user-friendly bioinformatics Read More...

The CCBR Single-cell RNA-seq Workflow on NIDAP Josh Meyer, bioinformatics analyst (CCBR), will cover a scRNA-seq workflow available to NCI researchers on NIDAP . NIDAP, the NIH Integrated Data Analysis Platform, is a cloud-based and collaborative Read More...

05/16/2024 - Qiagen CLC Genomics Workbench is a point-and-click bioinformatics software that runs on a personal computer and enables bulk RNA sequencing, ChIP sequencing, long reads, and variant analysis. NCI scientists can use CLC Genomics Workbench Read More...

03/05/2024 - This in-person workshop will show participants how to identify cell types based on statistics, visualization, and canonical markers. One Peripheral blood mononuclear cells (PBMCs) sample will be used to illustrate a basic Single Cell Read More...

11/16/2023 - This class will provide a demonstration of how to identify cell types based on statistics, visualization, and canonical markers. One Peripheral blood mononuclear cells (PBMCs) sample will be used to illustrate a basic Single Read More...

10/11/2023 - Partek Flow is your start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with analyzing Read More...

RNA-seq Bioinformatics Course from the Griffith lab.

Lesson 8: Introduction to RNA sequencing Slides

Lesson 12: RNA sequencing review 1 Learning objectives Here, we will do a quick review of what we have learned about RNA sequencing in Lessons 8 through 11. Accessing the Biostar handbook The URL for the Biostar handbook is Read More...

The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR Read More...

The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR Read More...

Overview of wet lab procedures Library preparation process, including Adapters and indices Single and paired end sequencing Strandedness Coverage and depth Spike-ins Replicates Batch effects Overview of analysis procedures References and annotation files needed for Read More...

RNA-SEQ Overview What is RNASEQ ? RNA-Seq (RNA sequencing), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment. (Wikipedia) Strictly speaking this could be any Read More...

Lesson 17: RNA sequencing review 2 Learning objectives This lesson will serve as comprehensive review of Module 2. We will spend roughly the first hour reviewing the Module 2 material the second hour answering specific questions from the poll Read More...

We will analyze these data by doing: 1. Alignment (hisat2) 2. Quantification (featureCounts) 3. Differential expression (DESeq) Use of a spike-in control Using a spike-in allows us to determine how well we can measure and reproduce data with Read More...

We will analyze these data by doing: 1. Alignment (hisat2) 2. Quantification (featureCounts) 3. Differential expression (DESeq) Use of a spike-in control Using a spike-in allows us to determine how well we can measure and reproduce data with Read More...

We will analyze these data by doing: 1. Alignment (hisat2) 2. Quantification (featureCounts) 3. Differential expression (DESeq) Use of a spike-in control Using a spike-in allows us to determine how well we can measure and reproduce data with Read More...

Thus far we have: Learned how to interact with our computer via command line. Downloaded raw RNA-Seq data (.fastq files). Examined raw data quality using fastqc and multiqc . Performed adapter and quality trimming using Trimmomatic . Read More...

Gene Expression Omnibus (GEO) (http://www.ncbi.nlm.nih.gov/geo/) Both microarray and sequencing data Sequence Read Archive (SRA) (http://www.ncbi.nlm.nih.gov/sra) All sequencing data (not necessarily RNA-Seq) ArrayExpress (https:// Read More...

The Central Dogma: Analyzing DNA, RNA, and Proteins

06/29/2023 - Starting with the classic Central Dogma of Molecular Biology, we will look at how each of the components (DNA, RNA, protein) is measured and analyzed. Next-Gen Sequencing (NGS) techniques, analyses tools available, and some Read More...

06/07/2023 - Join us for the upcoming CCR Fellows & Young Investigators-Seminar Series ( CCR FYI-SS 2022/2023 )  which is being held as a ‘ virtual ’ seminar that is open to everyone!  Julianna King , Laboratory Animal Sciences Program (LASP) “Genome-wide Read More...

03/30/2023 - Partek Flow provides a singular web based point and click environment for analyzing and visualizing high dimensional multi-omics sequencing data, making bioinformatics easily accessible to all researchers. Partek Flow software is available to NCI Read More...

10/21/2021 - RNA sequencing (RNA-seq) is a widely used method in genomics, which enables the interrogation of whole cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a RNA-seq workflow on the NIDAP collaboration platform, Read More...

10/07/2021 - RNA sequencing (RNA-seq) is a widely used method in genomics, which enables the interrogation of whole cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a RNA-seq workflow on the NIDAP collaboration platform, Read More...

09/23/2021 - RNA sequencing (RNA-seq) is a widely used method in genomics, which enables the interrogation of whole cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a RNA-seq workflow on the NIDAP collaboration platform, Read More...

09/02/2021 - RNA sequencing (RNA-seq) is a widely used method in genomics, which enables the interrogation of whole cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a RNA-seq workflow on the NIDAP collaboration platform, Read More...

08/19/2021 - RNA sequencing (RNA-seq) is a widely used method in genomics, which enables the interrogation of whole cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a RNA-seq workflow on the NIDAP collaboration platform, Read More...

08/05/2021 - RNA sequencing (RNA-seq) is a widely used method in genomics, which enables the interrogation of whole cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a RNA-seq workflow on the NIDAP collaboration platform, Read More...

03/06/2025 - This class introduces participants to the first steps to differential expression analysis in bulk RNA sequencing which involves filtering out noise from (ie. genes without expression across samples) and performing QC on the gene Read More...

12/12/2024 - This class will introduce bulk RNA sequencing analysis using Qiagen software. Participants will learn how to process FASTQ files and obtain differential expression using CLC Genomics Workbench as well as extract biological insight using Read More...

06/20/2024 - Dr. Irizarry will share findings demonstrating limitations of current workflows that are popular in single cell RNA-Seq data analysis. Specifically, he will describe challenges and solutions to dimension reduction, cell-type classification, and statistical significance Read More...

Josh Meyer, bioinformatics analyst (CCBR), will cover a scRNA-seq workflow available to NCI researchers on NIDAP . NIDAP, the NIH Integrated Data Analysis Platform, is a cloud-based and collaborative data aggregation and analysis platform that hosts Read More...

03/05/2024 - This in-person, hands-on training will introduce participants to single cell RNA sequencing analysis using Partek Flow, a point-and-click software for analyzing high dimensional multi-omics sequencing data. At NIH, Partek Flow is hosted on the Read More...

02/16/2024 - Deep sequencing has emerged as the primary tool for transcriptome profiling in cancer research. Like other high-throughput profiling technologies, sequencing is susceptible to systematic non-biological artifacts stemming from inconsistent experimental handling. A critical initial Read More...

10/25/2023 - Partek Flow is your start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with analyzing Read More...

09/27/2023 - Partek Flow is your start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with analyzing Read More...

09/13/2023 - Partek Flow is your start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with analyzing Read More...

{{Sdet}} Answer{{Esum}} Tools that we will be using for RNA sequencing analysis in this course series include command line applications for raw data quality assessment, data cleanup, trimming, alignment, etc. We will also be Read More...

Lesson 7: Downloading the RNA-Seq Data and Dataset Overview Lesson Review pwd (print working directory) ls (list) touch (creates an empty file) nano (basic editor for creating small text files) using the rm command to remove Read More...

Lesson 9: Reference genomes and genome annotations used in RNA sequencing Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 8 Review In Lesson 8, we learned about the basics of RNA sequencing, Read More...

This page contains content taken directly from the Biostar Handbook (Istvan Albert). Always remember to activate the class bioinformatics environment. conda activate bioinfo For this data analysis, we will be using: Two commercially available RNA Read More...

This page contains content taken directly from the Biostar Handbook (Istvan Albert). Always remember to activate the class bioinformatics environment. conda activate bioinfo For this data analysis, we will be using: Two commercially available RNA Read More...

Lesson 9: Reference genomes and genome annotations used in RNA sequencing Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 8 Review In Lesson 8, we learned about the basics of RNA sequencing, Read More...

Let's align an RNA-Seq sample using the "splice aware" aligner hisat2. First we will need to create the indices. Use this format: hisat2-build REFERENCE_GENOME INDEX_PREFIX Like this: hisat2-build Read More...

Let's align an RNA-Seq sample using the "splice aware" aligner hisat2. First we will need to create the indices. Use this format: hisat2-build REFERENCE_GENOME INDEX_PREFIX Like this: hisat2-build Read More...

Let's align an RNA-Seq sample using the "splice aware" aligner hisat2. First we will need to create the indices. Use this format: hisat2-build REFERENCE_GENOME INDEX_PREFIX Like this: hisat2-build Read More...

This page contains content directly from The Biostar Handbook . Always remember to start the bioinformatics environment. conda activate bioinfo Pseudoalignment-based methods identify locations in the genome using patterns rather than via alignment type algorithms. It Read More...

This page contains content directly from The Biostar Handbook . Always remember to start the bioinformatics environment. conda activate bioinfo Pseudoalignment-based methods identify locations in the genome using patterns rather than via alignment type algorithms. It Read More...

Lesson 16: RNA sequencing review and classification based analysis Before getting started, remember to be signed on to the DNAnexus GOLD environment. Review In the previous classes, we learned about the steps involved in RNA sequencing Read More...

06/14/2023 - Partek Flow is your start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with analyzing Read More...

04/26/2023 - In this month’s Cancer Genomics Cloud (CGC) webinar, Dr. Tolga Can of the Colorado School of Mines will share how he and members of the Erson-Bensan Lab are using publicly available Read More...

10/05/2022 - For our next CDSL webinar we will have presentations by two CDSL fellows:  Ekaterina Kazantseva  and Sanna Madan.  Ekaterina is a master’s student in Dr. Mikhail Kolmogorov's group and the title of Read More...

09/27/2022 - Course Objectives: This 12-week long course is for scientists wanting to learn basic bioinformatics skills. Material will be taught at a beginner to intermediate level. There are no pre-requisites. Learners will: Learn Unix skills Read More...

08/24/2022 - Meeting Link: https://cbiit.webex.com/cbiit/j.php?MTID=m875b987cd37fafd4c24a84b7296aadb0 This webinar will demonstrate new features for creating publication ready RNA-Seq Graphs using the easy Point-and-Click Read More...

06/29/2022 - THIS EVENT HAS BEEN RESCHEDULED FROM 6/22 TO 6/29 AT 11:00 AM USING THE SAME MEETING LINK. We will go over RNA-seq from experimental design,  data import options, data normalization options, to to statistical tests and biological Read More...

04/13/2022 - The first and the most common single cell application is single cell RNA-Seq, which enables scientists to focus on the gene expression profiles of individual cells rather than on tissue averages. Knowledge of expression Read More...

10/28/2021 - Single-cell RNA sequencing (scRNA-seq) is a relatively new and powerful method in genomics, which enables the interrogation of whole single cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a basic scRNA-seq workflow, Read More...

10/14/2021 - Single-cell RNA sequencing (scRNA-seq) is a relatively new and powerful method in genomics, which enables the interrogation of whole single cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a basic scRNA-seq workflow, Read More...

09/30/2021 - Single-cell RNA sequencing (scRNA-seq) is a relatively new and powerful method in genomics, which enables the interrogation of whole single cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a basic scRNA-seq workflow, Read More...

09/27/2021 - The NCI Genomic Data Commons’ (GDC) September webinar will introduce cancer researchers and bioinformaticians to the GDC's single-cell RNA-Seq (scRNA-Seq) data workflow. Single cell sequencing is a powerful platform for studying tumor heterogeneity Read More...

09/16/2021 - Single-cell RNA sequencing (scRNA-seq) is a relatively new and powerful method in genomics, which enables the interrogation of whole single cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a basic scRNA-seq workflow, Read More...

08/26/2021 - Single-cell RNA sequencing (scRNA-seq) is a relatively new and powerful method in genomics, which enables the interrogation of whole single cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a basic scRNA-seq workflow, Read More...

08/12/2021 - Single-cell RNA sequencing (scRNA-seq) is a relatively new and powerful method in genomics, which enables the interrogation of whole single cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a basic scRNA-seq workflow, Read More...

Clinical Support Laboratory – Cellular Immunology offers a spectrum of bioassays to assess cellular responses to antigens and mitogens including ELISPOT, cytotoxicity assays, and proliferation assays. Established Technologies Elispot Assays, Dual or Triple Color Fluorospot Assays, Read More...

The Genomics Technology Laboratory is an integrated, high-throughput molecular biology laboratory focusing on the development of genetics and genomics technologies, data analysis, and information management tools, in support of CCR Investigators. The laboratory develops integrated Read More...

NISC’s role within NHGRI, and more broadly across NIH, aims to advance genome sequencing and its many applications, with a goal not simply to produce sequence data, but to produce the infrastructure required to Read More...

The CCR Genomics Core is located in Building 41 on the NIH Bethesda campus. The primary goal of the Core is to provide investigators from CCR/NCI and other NIH Institutes access to genomic technologies and Read More...

The NIA Nonhuman Primate (NHP) Core Facility maintains a colony of rhesus macaques at the National Institutes of Health Animal Center. Established in 2012, the Core's mission is to offer research services supporting multi-disciplinary translational aging Read More...

The National Cancer Institute (NCI) is developing a national repository of Patient-Derived Models (PDMs) comprised of patient-derived xenografts (PDXs), patient-derived organoids (PDOrg), and in vitro patient-derived tumor cell cultures (PDCs) and cancer-associated fibroblasts (CAFs). These Read More...

Biomarker Discovery with Morphological Context:  Changing how tissue specimens are analyzed < https://youtu.be/mVhfZq8ppbc What is Digital Spatial Profiling? GeoMx Digital Spatial Profiler is a novel platform developed by NanoString. Digital Spatial Read More...

The Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Established Technologies Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), Intracellular markers, including cytokines and Read More...

The PPS encompasses all scientific analyses related to pharmacology, once the specimen has been collected and stored. There is a multi-step process to evaluate how the drug is being handled by the body after administration. Read More...

The Office of Collaborative Biostatistics, Office of the Clinical Director, is the statistical and data management component of CCR. The Section provides statistical leadership and data management consultation for CCR’s major clinical activities and Read More...

NCI maintains a low temperature repository of implantable in vivo-derived tumors and in vitro-established tumor cell lines from various species, which are available to qualified investigators as a service to the cancer research community. The Read More...

Employing spatial biology techniques enables acquisition of transcript and protein data from intact tissue sections, and in turn, spatial distribution information and cellular interaction patterns are revealed.

Biophysics Core Facility assists NIH investigators in measuring molecular interactions and in characterizing macromolecular properties. This includes binding studies of proteins, DNA, RNA, and their ligands in buffers, cell lysate, plasma, and other media. We Read More...

CREx News & Updates November 2021 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight FACILITY HIGLIGHTS Learn more about services from the NINDS Quantitative Magnetic Resonance Imaging Core. NINDS Read More...

The mission of Human Brain Collection Core (HBCC) within the National Institute of Mental Health, Division of Intramural Programs (NIMH IRP) is to conduct and support research on brain and behavior, with the goal of Read More...

October 6, 2022 crex.nih.gov CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight NIH Center For Human Immunology, Inflammation, And Autoimmunity (CHI) CHI is a Read More...

NCI established the Nanotechnology Characterization Laboratory (NCL) to support the extramural research community to accelerate the progress of nanomedicine by providing preclinical characterization and safety testing of nanoparticles. It is a collaborative effort between NCI, Read More...

September 8, 2022 crex.nih.gov CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight Trans-NIH Intramural Sequencing Center (NISC) NISC is a multi-disciplinary genomics facility that Read More...

The NIH Library is a biomedical research library, whose collection and services are available at no cost to NIH staff: Electronic Resources: Over 20,000 electronic journals, 190,000 eBooks, and 50 databases. , Training classes covering topics such as bioinformatics, Read More...

The Mass Spectrometry Unit of the Collaborative Protein Technology Resource uses mass spectrometry for the study of proteins. We perform collaborative experiments with CCR researchers in areas such as: interactomes, quantitative global proteome analyses, and Read More...

The goals of the Bioinformatics Training and Education Program within NCI/CCR are: To make researchers aware of the bioinformatics resources available to them, To provide training and guidance on these resources regularly and at Read More...

Our operational objectives are to provide state-of-the-art OMICS technologies in support of the Genetics Branch (GB) investigators and collaborators. Research Services Wet Lab Single cell isolation from fresh, frozen, and FFPE tissue, DNA/RNA extractions Read More...

The introduction of DNA sequencing instruments capable of producing millions of DNA sequence reads in a single run has profoundly altered the landscape of genetics and cancer biology. Complex questions can now be answered at Read More...

The NCI Genomic Data Commons (GDC) was established by the NCI Center for Cancer Genomics (CCG) to support the receipt, harmonization, distribution, and analysis of genomic and clinical data from cancer research programs. The GDC Read More...

Protein Characterization Laboratory (PCL) offers various technologies to CCR investigators to characterize proteins and metabolites. The laboratory develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, liquid chromatography, and Surface Plasmon Resonance (SPR), to advance Read More...

We are a bioinformatics team within the Center for Biomedical Informatics and Information Technology’s (CBIIT’s) Cancer Informatics Branch (CIB)—soon to be referred to as the Informatics and Data Science (IDS) Program. Headed Read More...

In order to meet increasing demands from both NIH intramural and extramural communities for access to a small angle X-ray scattering (SAXS) resource, the Center for Cancer Research (CCR) under the leadership of Drs. Jeffrey Read More...

CLIA-Certified Technologies Offered: Fragment Analysis for Micro-satellite Instability Detection, Pharmacoscan Array for Pharmacogenomics, Mutation Detection for PCR and Sanger Sequencing, DNA extraction from whole blood, saliva, FFPE tissues, buccal swabs, nails, hair, PBMCs, buffy coats, Read More...

Protein Expression Laboratory The Protein Expression Laboratory develops, improves, and delivers protein-centric services. Our goal is to help client investigators achieve their research goals with the lowest possible cost in the shortest time. All PEL Read More...

What is Xenium? Xenium is a high-resolution, imaging-based in situ spatial profiling technology from 10x Genomics that allows for simultaneous expression analysis of RNA targets (currently in range of 100’s) within the same tissue section. Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New NIH Resource Resources Advance your research with the NIH Mouse Imaging Facility (MIF) The NIH Mouse Imaging Read More...

Back Services: Biophysics Facility offers MP as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument training calendar.  Training includes mass distribution analysis of a Read More...

The Bloomington Drosophila Stock Center (BDSC) collects, maintains, and distributes genetically defined strains of Drosophila melanogaster for research and education. The BDSC supports a large, worldwide community of scientists using Drosophila as a model organism Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More. New Resources on CREx NIMH Human Brain Collection Core (HBCC) The HBCC obtains human brain tissue, hair, and blood Read More...

The NIH Center for Human Immunology, Inflammation, and Autoimmunity (CHI) is a trans-NIH resource whose mission is to provide a collaborative hub of advanced translational immunology for NIH clinical and pre-clinical studies. This uniquely structured Read More...

The Medicinal Chemistry Accelerator (MCA) is a collaborative CCR resource that supports investigators in developing small molecule inhibitors for anticancer drug candidates. While CCR and NCATS have infrastructure to identify initial “hits” through high-throughput screening, Read More...

CREx News & Updates January 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight NCI ANTIBODY CHARACTERIZATION LABORATORY (ACL) The ACL specializes in rigorously validating antibodies for signaling and Read More...

Thousands of molecular targets have been measured in the NCI panel of 60 human tumor cell lines. Measurements include protein levels, RNA measurements, mutation status, and enzyme activity levels. You can choose to search for a Read More...

Home About the Biophysics Core Biophysics Core Services [tabby title="Instrumentation"] NHLBI Biophysics Core The Biophysics Core Facility: Overview Core Facilities provide scientific resources, cutting-edge technologies and novel approaches to support DIR scientists. Availability of Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Technology Event Biophysical Methods for Protein Interactions Monday, May 15 – Friday, May 19, 2023 This workshop will review the strategies of Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New NIH Resource Spotlight The NIH Lab Managers Working Group have developed a new NIH-wide database of cold Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New NIH Resource Resources Derive Greater Insights and Accelerate your Research Using Bioinformatic Tools! CREx is an NIH Read More...

Protein Characterization Laboratory (PCL) offers various technologies to CCR investigators to characterize proteins and metabolites. The laboratory develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, liquid chromatography, and Surface Plasmon Resonance (SPR), to advance Read More...

The Center for Advanced Preclinical Research (CAPR) specializes in evaluating the efficacy of preclinical compounds, existing drugs, or biologics (therapeutics) in genetically engineered mouse models, GEM-derived allograft (GDA) models, or patient-derived mouse xenografts (PDX). We Read More...

The Mouse Modeling Core assists NIH investigators by generating and preserving genetically-engineered mouse strains. Services include scientific consultation, gene-targeting in mouse embryonic stem cells, micro-injection of nucleic acids, proteins, or ES cells into mouse embryos, Read More...

What is Visium FFPE v2 with CytAssist? Visium FFPE v2 is sequencing-based spatial profiling technology developed by 10x Genomics. This assay can take mouse or human tissue sections on normal glass slides as input and Read More...

The Genomics Laboratory (formerly Laboratory of Molecular Technology) is an integrated, high-throughput molecular biology laboratory focusing on the development of genetics and genomics technologies, together with associated laboratory automation systems, data analysis, and information management Read More...

The NCI High-Throughput Imaging Facility (HiTIF) works in a collaborative fashion with NCI/NIH Investigators by providing them with the necessary expertise, instrumentation, and software to develop and execute advanced High-Throughput Imaging (HTI) assays. These Read More...

The Biophysics Core’s mission is to provide support in the study of macromolecular interactions, dynamics, and stability by offering consultations, training, professional collaborations, and instrument access. General Services Multi-technique molecular interaction studies, Kinetic and Read More...

Back Services: Biophysics Facility offers ITC calorimeters as open-access instruments.  First-time users must complete a short training session before gaining access to the instrument reservation calendar.  Training includes performing a test experiment and Read More...

The Clinical Support Laboratory offers processing, tracking, and testing of a broad range of clinical samples. Support can begin at the early stages of clinical trial development to aid in developing a comprehensive strategy for Read More...

Supplemental Technology Award Review System (STARS) Overview STARS Request Form STARS System The Supplemental Technology Award Review System (STARS) is a web-based interface for submission and review of S&S supplement requests by CCR Read More...

The facilities at AIM are available for use by the entire NIH intramural research community. While we welcome users with any size imaging project, AIM specializes in large, yearlong (or longer), collaborative research efforts with Read More...

Back Services: Biophysics Facility offers MST as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument reservation calendar.  Training includes the KD determination of a Read More...

The Genomics and Pharmacology Facility is part of the NCI's Center for Cancer Research (CCR), within the Developmental Therapeutics Branch. Its mission is to manage and assess molecular interaction data obtained through multiple platforms, increase Read More...