Search Results for: Design

nano design.csv Copy the text below to the nano editor, hit control-x and save to return to the terminal. sample,condition BORED_1.bam,BORED BORED_2.bam,BORED BORED_3.bam,BORED EXCITED_1.bam,EXCITED EXCITED_2. Read More...

11/15/2024 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...

12/04/2023 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...

06/27/2023 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...

03/08/2023 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This five-part lecture series will Read More...

09/30/2022 - The goal of this webinar series is to enhance understanding within the NCI and the research community of opportunities for targeting fusion oncoproteins through emerging chemoproteomic methods. This presentation will focus on computational tools Read More...

05/18/2022 - During the May Accelerating Therapeutics for Opportunities in Medicine (ATOM) Webinar Series, discover how computing and machine learning can accelerate molecular optimization for cancer and infectious disease therapeutics. The  ATOM Consortium  is a public-private Read More...

04/29/2021 - BTEP_April29_2021 Slides Recording Experimental Design Considerations in Variant Analysis Germline vs Somatic WGS vs WES Sample sizes and statistical power WGS/WES Pipelines at NIH Pipeline performance Using Pipeliner for internal and external Read More...

04/25/2017 - This talk will be an introduction to Methyl-seq, where you will learn about leveraging the power of next-generation sequencing (NGS), both genome-wide and targeted approaches, that can provide insight into methylation patterns at a Read More...

07/23/2020 - A panel of scientists with expertise in Single-Cell RNA-Seq will answer your questions about Single-Cell RNA-Seq experimental design. Please submit your question by July 15, to https://btep.ccr.cancer.gov/question/single_cell_rna_ Read More...

03/10/2025 - AI Club is a weekly meeting that explores various topics relating to AI and deep learning in biomedical sciences, typically in a seminar, workshop, or journal club format. AI Club is intended to be Read More...

Notes: linux - how to get help (man, flags) file permissions - changing permissions include grep and regular expressions

Notes: linux - how to get help (man, flags) file permissions - changing permissions include grep and regular expressions

01/13/2020 - Debra S. Marks, PhD Principal Investigator Associate Professor, Harvard Medical School

01/23/2017 - The Bioinformatics Training and Education Program (BTEP), Office of Science and Technology Resources (OSTR) is excited to announce a series of workshops focused on the analysis of next-generation sequencing (NGS) data. The in-depth, comprehensive Read More...

03/26/2024 - Dear Colleagues,   Developed by expert statisticians and programmers, SUDAAN is a software package designed for researchers who work with study data. SUDAAN procedures properly account for correlated observations, clustering, weighting, stratification, and Read More...

Let's now create a gene expression heatmap for results generated using the classification based approach. We have our results.csv and design.csv file in our salmon directory so we just need to do Read More...

Can we generate an expression heatmap? {{Sdet}} Solution{{Esum}} Rscript $CODE/create_heatmap.r {{Edet}} Next, let's generate the Principal Components Analysis plot. But first, we need to convert the counts.csv and design. Read More...

04/10/2025 - The cost of handling rare and/or late-stage diseases far exceeds preventative strategies. Cell and gene therapies can be quite cost-effective when applied at large population scales (e.g. pandemic vaccines and transfusions).   AI Read More...

03/17/2025 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering a several trainings that cover general concepts behind statistics and epidemiology. These trainings will help Read More...

Bioinformatics support to CCR investigators is provided by: CCR Collaborative Bioinformatics Resource (CCBR) The CCR Collaborative Bioinformatics Resource (CCBR) is a resource group which provides a mechanism for CCR researchers to obtain many different types Read More...

10/10/2024 - This introductory lecture will provide an overview of bulk RNA-Seq technology, its various application and shortcomings, as well as detailing the steps involved in analyzing the resulting data. The talk is aimed at those Read More...

The Single Cell Analysis Facility (SCAF) is a CCR facility dedicated to the application of single-cell technologies in cancer research. Based on the NIH Bethesda main campus, SCAF aims to provide the broadest range of Read More...

04/03/2024 - The Single Cell Analysis Facility (SCAF) is a CCR facility dedicated to the application of single-cell technologies in cancer research. Based on the NIH Bethesda main campus, SCAF aims to provide the broadest range Read More...

The tidyverse is an opinionated collection of R packages designed for data science. All packages share an underlying design philosophy, grammar, and data structures. --- tidyverse.org The core packages within tidyverse include dplyr , ggplot2 , Read More...

Lastly, the QIIME2 forum is a fantastic resource to get help with qiime2 plugins or questions related to your workflow or research design. There is also a "best-of-the-forum" tag, which is worth a Read More...

The object class used by the DESeq2 package to store the read counts and the intermediate estimated quantities during statistical analysis is the DESeqDataSet. --- Analyzing RNA-seq data with DESeq2 Constructing this object from a Read More...

Next, we need to generate the counts (ie. number of reads that map to a transcript). But first, change back into the ~/biostar_class/snidget folder and then take a moment to think about how Read More...

Central Dogma of Molecular Biology What is RNA Sequencing? How to Design an RNA-Seq Experiment Biological and Technical Replicates

Remember: Replicates, batch effects, consult BEFORE sequencing CCBR Best Practices for Experimental Design Sequence depth and machine requirements estimates can be obtained from the Illumina Sequencing Coverage Calculator Cost estimates can be previewed at the Read More...

After the merged expression counts table has been created, we can proceed with differential expression analysis. Let's use DESeq2 again for this. But first, let's move counts.csv (the merged salmon expression table) Read More...

"The goal of DAVID's design is to be able to efficiently upload and analyze a list consisting of

"The goal of DAVID's design is to be able to efficiently upload and analyze a list consisting of

Lesson 16 Practice Objectives In this lesson, we learned about the classification based approach for RNA sequencing analysis. In this approach, we are aligning our raw sequencing reads to a reference transcriptome rather than a genome. Read More...

Prior to differential expression analysis, we need to generate a design.csv file that contains the samples and their corresponding treatment conditions. Note that csv stands for comma separated value so the columns in these Read More...

Plots condense complex and busy tabular data into a form that is easier to interpret. An expression heatmap is a common visualization used in RNA sequencing analysis. A heatmap shows numerical data on a color Read More...

A typical RNASEQ experiment involves several steps, only one of which falls within the realm of bioinformatics. Namely the Data Analysis step. Experimental Design What question am I asking How should I do it (does Read More...

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No summary available.

MAB provides visual communication solutions across all media to the entire NIH community. Our Medical Illustration section provides a complete range of biomedical visualization services, including manuscript/textbook figures, infographics, 3D modeling, animation, technical diagrams, Read More...

Wide-field microscopy This is the technical term for a conventional fluorescence microscope. Typically the entire field of view of the specimen is illuminated with excitation light and then all of the emitted fluorescence is collected Read More...

Our Mission The NCI Optical Microscopy Cores are instrumental in advancing cancer research through optical measurements and analysis. Serving the Center for Cancer Research community, which is home to approximately 250 Principal and Senior Investigators across 50 Read More...

Our Team Tatiana S. Karpova Ph.D.Core Headkarpovat@nih.govBuilding 41, Room C615240-760-6637 David A. Ball Ph.D.Core Biologistballa@nih.govBuilding 41, Room B114D240-760-6577 Mohamadreza Fazel, Ph.D.Core Biologistmohamadreza. Read More...

A typical HTI screening project at HiTIF can be subdivided in 5 phases: Pre-Development The investigator contacts the Facility Head to inquire about the current availability of instrumentation time and manpower for the project to be Read More...

07/31/2025 - NIDDK Biostats Seminar Series: From Research Study Design to Collecting, Managing, and Analyzing Data. Learning Objectives: Be able to identify, load, and use R resources/packages based upon needs and experience level with R.& Read More...

07/24/2025 - NIDDK Biostats Seminar Series: From Research Study Design to Collecting, Managing, and Analyzing Data. Learning Objectives: 1.          To delineate Read More...

04/28/2025 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering a several trainings that cover general concepts behind statistics and epidemiology. These trainings will help Read More...

The computational chemistry and protein modeling team in the Advanced Biomedical Computational Science (ABCS) group provides novel solutions in structural modeling and computational chemistry. Computational scientists in the group collaborate with NCI researchers by using Read More...

01/08/2025 - This three hour online training will focus on getting started on using QIAGEN’s  Ingenuity Pathway Analysis (IPA) . IPA enables you to quickly identify biological relationships, mechanisms, pathways, functions and Read More...

12/09/2024 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...

12/03/2024 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...

11/13/2024 - The National Library of Medicine (NLM) Division of Intramural Research (DIR) is pleased to welcome Manisha Desai, PhD, Section Chief of Biostatistics and Director of the Quantitative Sciences Unit at Stanford University School of Read More...

11/08/2024 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...

Congratulations, your experiments have been completed and you have a large amount of data. Now, it’s time to analyze these data. But where do you begin? How can you gain the most meaning from Read More...

The CCR Single Cell Analysis Facility (SCAF): An Overview The Single Cell Analysis Facility (SCAF) is a CCR facility dedicated to the application of single-cell technologies in cancer research. Based on the NIH Bethesda main Read More...

In this tutorial, we will continue to use data from Nanduri et al. 2022, Epigenetic regulation of white adipose tissue plasticity and energy metabolism by nucleosome binding HMGN proteins , published in Nature Communications . As a reminder, Read More...

In this tutorial, we will continue to use data from Nanduri et al. 2022, Epigenetic regulation of white adipose tissue plasticity and energy metabolism by nucleosome binding HMGN proteins , published in Nature Communications . As a reminder, Read More...

05/15/2024 - In this one-hour webinar, you'll get a demonstration of DNASTAR Lasergene Software. DNASTAR offers software solutions for molecular biology, protein analysis, and genomics. This presentation will focus on an overview of the applications Read More...

05/08/2024 - Qlucore Omics Explorer is a point-and-click software that enables analysis of RNA sequencing (bulk and single cell), proteomics and metabolomics data. It’s machine learning capabilities also allow for classification of cell types. This Read More...

The Bioinformatics Training and Education Program (BTEP) was established by the Office of Science and Technology Resources (OSTR) for the purpose of providing training enabling scientists to understand and analyze their own experimental data, at Read More...

12/18/2023 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...

S4 is a rigorous system that forces you to think carefully about program design. It’s particularly well-suited for building large systems that evolve over time and will receive contributions from many programmers. --- Advanced Read More...

For the help sessions, we will work on processing sequences generated in Zhang Z, Feng Q, Li M, Li Z, Xu Q, Pan X, Chen W. Age-Related Cancer-Associated Microbiota Potentially Promotes Oral Squamous Cell Cancer Read More...

Let's remember back to the design of the study we are examining ( Reconstitution of the gut microbiota of antibiotic-treated patients by autologous fecal microbiota transplant ). This study included a randomized controlled longitudinal trial involving 25 Read More...

Work through DNAnexus? Find a nice cancer data set for 16S and shotgun Divide into two workshops or courses? Course structure or workshop structure? Amplicon processing and analysis (5 week course) (4 weeks) 1. Talk by Greg Caparaso? 1. Read More...

Microbiome Course Course Outline Work through DNAnexus? Find a nice cancer data set for 16S and shotgun Divide into two workshops or courses? Course structure or workshop structure? Amplicon processing and analysis (5 week course) (4 weeks) 1. Read More...

The geom functions require a mapping argument. The mapping argument includes the aes() function, which "describes how variables in the data are mapped to visual properties (aesthetics) of geoms" (ggplot2 R Documentation). If Read More...

12/12/2023 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...

12/08/2023 - This presentation will discuss strategies and policies for effective sharing and reuse of large multidimensional datasets. Dr. Espinosa will discuss his experiences as a data generator, data analyst, collaborator, teacher, and mentor through the Read More...

12/06/2023 - This presentation will provide a one-hour overview demonstration on Geneious Prime, a software platform for molecular biology and sequence analysis. It is built to be highly visual, easy to use, and collaborative. & Read More...

11/28/2023 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...

11/21/2023 - Dear Colleagues,    This presentation will provide an overview of OpenCRAVAT.   Annotation and interpretation of cancer variants is critical to the design of personalized molecular therapies, and Read More...

09/20/2023 - This session of the BTEP Coding Club will focus on the tool rMATS for differential alternative splicing event detection from RNA-Seq data. This 1-hour demo will provide a detailed overview of rMATS including why Read More...

Lesson 15 Practice Objectives Previously, we performed QC on the Golden Snidget RNA sequencing data, aligned the sequencing reads to its genome, and obtained expression counts. We can now finally perform differential expression analysis, to find Read More...

Statistical Models - these should be appropriate for your experimental design. The methods you are using should be consistent with what's in related scientific literature. Different tests produce different results. Consult a statistician if Read More...

Before we can align the HBR and UHR raw sequencing data to human chromosome 22 transcriptome, we need to create an index of this transcriptome (like we did with the genome). This will make the alignment Read More...

Lessons focus on RNA-Seq analysis including experimental design and best practices, quality control, trimming, alignment based methods, classification based methods, feature counts, and differential expression analysis. Lesson 8: Introduction to RNA-Seq ( Recording ) Lesson 9: Introduction to the Read More...

Course setup Intro to DNAnexus and the GOLD learning system Learning Objectives Unix Bootcamp Brief Review of R (for R scripts in later analyses) Introduction to RNA Sequencing Central Dogma of Molecular Biology What is Read More...

RNA-SEQ Overview What is RNASEQ ? RNA-Seq (RNA sequencing), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment. (Wikipedia) Strictly speaking this could be any Read More...

Biostar Class - Sequencing Instruments Below are a list of links and resources mentioned in the BIOSTAR Sequencing Instruments class given on 06/10/20 and 06/11/20 Sequencing Technologies - Company Web Sites Illumina PacBio Oxford Nanopore 10X Genomics Read More...

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No summary available.

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The research conducted within the Synthetic Biologics Core (SBC) Facility has a dual role: Generate chemical biology tools and drug candidates for molecular targets identified by NCI research groups, Develop novel effective methods and tools Read More...

NCI LASP Genome Modification Core (GMC) is a CCR-dedicated facility that provides advice, training, and reagents to NCI scientists seeking to utilize CRISPR and other nucleases to generate genome modifications in primary cells, cell lines, Read More...

Employing spatial biology techniques enables acquisition of transcript and protein data from intact tissue sections, and in turn, spatial distribution information and cellular interaction patterns are revealed.

NanoString Technology The nCounter® Analysis System is an automated, multi-application, digital detection and counting system which directly profiles up to 800 molecules simultaneously from a single sample using a novel barcoding technology. Profile hundreds of mRNAs, Read More...

The Medicinal Chemistry Accelerator (MCA) is a collaborative CCR resource that supports investigators in developing small molecule inhibitors for anticancer drug candidates. While CCR and NCATS have infrastructure to identify initial “hits” through high-throughput screening, Read More...

The Biophysics Resource (BR) was established in January 2001. Our mission is to provide CCR investigators with access to both the latest instrumentation and expertise in characterizing the biophysical aspects of systems under structural investigation. The Read More...

.row { display: flex; justify-content: space-around; align-items: flex-start; margin: 20px; } .column { text-align: center; padding: 10px; width: 30%; } .column img { display: block; margin: 0 auto; width: 150px; height: 150px; } .column strong { display: block; margin-top: 10px; } Background: Intravital microscopy (IVM) Read More...

The Antibody Characterization Laboratory (ACL) is the laboratory responsible for the development of well-characterized monoclonal antibody reagents. The NCI’s Office of Cancer Clinical Proteomics Research funds ACL as a resource to the entire cancer Read More...

The core provides access to several different state-of-the-art 3D microscopes as well as computers to visualize and process image data. The facility houses equipment for 2D or 3D imaging of fixed and living specimens. High Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Intramural Core Resources NHGRI Flow Cytometry Core The Core performs high quality single cell analysis through the Read More...

The Office of Collaborative Biostatistics, Office of the Clinical Director, is the statistical and data management component of CCR. The Section provides statistical leadership and data management consultation for CCR’s major clinical activities and Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New CREx Program Manager The CREx Team is happy to announce Deepika Velampati as the new CREx Program Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New CREx User Survey The CREx Team is carrying out a CREx User Survey. We would greatly appreciate Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New NIH Resource Resources Advance your research with the NIH Mouse Imaging Facility (MIF) The NIH Mouse Imaging Read More...

The Mid-Atlantic CHTN is one of six participating divisions of the Cooperative Human Tissue Network (CHTN) and is based in the Pathology Department at the University of Virginia. While primarily a resource for cancer researchers, Read More...

Electron Microscopy Laboratory (EML) The EML offers investigators access to unique expertise and EM technologies that allow our partners to explore new avenues of research to enhance the knowledge of biological systems. To assist our Read More...

The EML offers investigators access to unique expertise and EM technologies that allow CCR Investigators to explore new avenues of research in order to enhance the knowledge of biological systems. To assist our customers, we Read More...

The Center for Inherited Disease Research (CIDR)'s  mission is to support the genetics community by providing high-quality, cutting-edge genomics services and technologies in order to expand our understanding of disease and catalyze discoveries that Read More...

The Metabolic Clinical Research Unit (MCRU) opened in 2007 as a key component of the Strategic Plan for NIH Obesity Research . The NIDDK worked closely with the Clinical Center to design the Unit, which houses state-of-the-art Read More...

The NCI Clinical Research Correlatives Core provides non-CLIA-certified spectral flow cytometric assays to support clinical trials conducted in the CCR. The core specializes in immunophenotyping and immune monitoring assays. Established Technologies Spectral flow cytometry (Cytek), Read More...

The NIH Clinical Center Pharmacy Department provides pharmaceutical care to inpatients and outpatients on NIH intramural research protocols at the NIH Clinical Center. The Clinical Center facility encompasses 200 inpatient beds, 93 day-hospital stations, and 15 clinics. Clinical Read More...

The rapid advancement of single-cell technology has provided new powerful tools to answer many biological questions, such as identifying new or rare cell populations and characterizing the complexities of tumor heterogeneity. Realizing the great potential Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Research Festival The NIH Research Festival highlights the groundbreaking science and the vibrant NIH community driving our Read More...

NIH Intramural CryoEM Consortium (NICE) serves intramural investigators in all NIH IC’s. NICE provides access to state-of-the-art Titan Krios cryo-electron microscopes for atomic-resolution structure determination of protein, macromolecular complexes, membrane receptors, cellular organelles, and Read More...

September 8, 2022 crex.nih.gov CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Science & Technology Seminars and Training Events Upcoming Seminars and Educational Opportunities The following Read More...

The Staff Scientists/Clinicians (SSSC) Technical Enrichment Program (STEP) was established to provide SSSC’s an opportunity to compete for funding to gain comprehensive training in state-of-the-art techniques available through CCR Cores and Facilities. The Read More...

NCI LASP Animal Research Technology Support (ARTS) provides customized technical support for basic and translational animal-based research to the scientific community. We offer a wide array of services ranging from expert colony management to the Read More...

The centrally funded Statistics team within the Advanced Biomedical Computational Science group at the Frederick National Lab provides statistical consultation and data analysis support for NCI laboratories. We have broad-range expertise in biomedically relevant areas Read More...

Large STARS can be used to request supplemental funding exceeding $8,000 to offset experimental costs (e.g., core or vendor services or specialized reagents).  Applications require detailed scientific and budgetary justification and must be proposed in Read More...

The introduction of DNA sequencing instruments capable of producing millions of DNA sequence reads in a single run has profoundly altered the landscape of genetics and cancer biology. Complex questions can now be answered at Read More...

The CCR Genomics Core is located in Building 41 on the NIH Bethesda campus. The primary goal of the Core is to provide investigators from CCR/NCI and other NIH Institutes access to genomic technologies and Read More...

What is Xenium? Xenium is a high-resolution, imaging-based in situ spatial profiling technology from 10x Genomics that allows for simultaneous expression analysis of RNA targets (currently in range of 100’s) within the same tissue section. Read More...

The FNLCR Molecular Histopathology Laboratory (MHL) provides comprehensive veterinary pathology support for animal health monitoring, biomarker discovery and validation, drug development, genomics, and proteomics on a cost recovered basis. The MHL is organized into multiple Read More...

The CCR Building 41 Flow Cytometry Core is a full-service facility within the Center for Cancer Research that supports over 150 users representing 26 laboratories. The Core Facility provides instrument and software training, technical expertise, assay development, and Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New NIH Resource Resources Derive Greater Insights and Accelerate your Research Using Bioinformatic Tools! CREx is an NIH Read More...

The Genomics Laboratory (formerly Laboratory of Molecular Technology) is an integrated, high-throughput molecular biology laboratory focusing on the development of genetics and genomics technologies, together with associated laboratory automation systems, data analysis, and information management Read More...

OMAL focuses its research and development activities to quantitatively understand the molecular basis of three-dimensional (3D) cell organization, motility, invasion, and differentiation using fixed samples and live, 3D tissue culture models (i.e., translational models). Read More...

The CCR Collaborative Bioinformatics Resource (CCBR) is a centrally funded resource group which provides a mechanism for CCR researchers to obtain many different types of bioinformatics assistance to further their research goals. The group has Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New NIH Resource Spotlight The NIH Lab Managers Working Group have developed a new NIH-wide database of cold Read More...

The Biomedical Engineering and Physical Science (BEPS) shared resource supports NIH’s intramural basic and clinical scientists on applications of engineering, physics, imaging, measurement, and analysis. BEPS is centrally located on the main NIH campus Read More...

The Chemistry and Synthesis Center (CSC) of the National Heart, Lung, and Blood Institute (NHLBI) provides IRP scientists with targeted imaging probes and chemical tools that help accelerate cell-based assays, in vivo imaging studies, and Read More...

CREx News & Updates July 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Click below to learn how easy it is to navigate the CREx platform. These short videos will Read More...

The NIH Center for Human Immunology, Inflammation, and Autoimmunity (CHI) is a trans-NIH resource whose mission is to provide a collaborative hub of advanced translational immunology for NIH clinical and pre-clinical studies. This uniquely structured Read More...

The Spatial Imaging Technology Resource (formerly the Nanoscale Protein Analysis Section of the Collaborative Protein Technology Resource or CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery Read More...

Our operational objectives are to provide state-of-the-art OMICS technologies in support of the Genetics Branch (GB) investigators and collaborators. Research Services Wet Lab Single cell isolation from fresh, frozen, and FFPE tissue, DNA/RNA extractions Read More...

[tabby title="Home"] About NICE-NIH Intramural CryoEM Consortium  NIH Intramural CryoEM Consortium (NICE) serves intramural investigators in all NIH IC’s. NICE provides access to state-of-the-art Titan Krios cryo-electron microscopes for atomic-resolution structure determination of Read More...

In order to meet increasing demands from both NIH intramural and extramural communities for access to a small angle X-ray scattering (SAXS) resource, the Center for Cancer Research (CCR) under the leadership of Drs. Jeffrey Read More...

Multicolor imaging Fluorescence imaging is used for protein localization and colocalization in 3D. Multi-color imaging is necessary to observe colocalization of several proteins in the same cell. Many fluorescent proteins are now available for multi-color Read More...

Training Contact the LCBG Microscopy Core to discuss your experiment: Meet with Ross Lake, Core Head, to discuss your specific project. If the project is feasible, schedule an appointment to receive training and use the Read More...

Techniques The Laboratory of Cancer Biology and Genetics Microscopy Core houses multiple systems that can be used to analyze cell structure, protein expression, and cell dynamics using immunofluorescence. These include inverted epifluorescence microscopes, a confocal Read More...

Software Image Acquisition Commercial imaging systems of LRBGE Optical Microscopy Core are controlled by acquisition software specifically designed for the appropriate microscope, such as ZEN (Zeiss confocal microscopes), Nikon Elements (Nikon), Imspector (Abberior). Custom-built HILO Read More...

General Usage Policies Principal investigators should read this document and sign it. PI’s/postdocs should attach a short-written summary of the project to the signed doc. If the project changes, Read More...

Software LAS Leica Application Suite X (LAS X) is the one software platform for all Leica microscopes: It integrates confocal, wide field, stereo, super-resolution, and light-sheet instruments from Leica Microsystems. MetaMorph The MetaMorph® Microscopy Automation Read More...

Macro’s for Image Processing Dr. Wisniewski wrote a number of custom ImageJ Macros aimed at streamlining image processing tasks such as: Software Imaris The world’s leading Interactive Microscopy Image Analysis software company, actively Read More...

Image Analysis Signals Imaging Artist Next generation image analysis and management platform for high-content screening and cell imaging data. Quickly process, analyze, share, and store the vast volumes of data. Cell Profiler CellProfiler is free, Read More...

Stephen Lockett, Ph.D. Director, OMAL locketts@nih.gov 301-846-5515 Valentin Magidson, Ph.D. Scientist magidsonv@mail.nih.gov 301-846-6092 Will Heinz, Ph.D. Scientist heinzwf@nih.gov 301-846-1239 David Read More...

Research Mission The goal of OMAL’s research is to understand molecular mechanisms driving carcinogenesis and the reversal of this process through treatment, by utilization and advancement of optical microscopy techniques. These techniques include multiplex Read More...

BookIt The NCI Microscopy Core is using a new system to reserve a microscope. Use this link to access the BookIt software. Click on the NIH Login to sign in using your NIH PIV ID Read More...

Software Several software packages are available to users Arivis Vision 4D Arivis Vision4D is a modular software for working with multi-channel 2D, 3D and 4D images of almost unlimited size independent of Read More...

Most BTEP courses include detailed course materials including lesson content, additional resources, and lesson associated data. These course materials are listed here so that learners can easily return to and review concepts taught in class Read More...

General questions or comments about the BTEP program or classes should be addressed to: NCIBTEP@nih.gov Desiree Tillo, Ph.D . Staff Scientist desiree.tillo@nih.gov I have a broad research background in bioinformatics Read More...

Listed below are the video recordings of past BTEP events (classes, seminars, workshops). Videos are hosted on various servers and may play slightly differently. Some videos may be downloaded for local viewing. Recorded Videos of Read More...

What is bioinformatics? Bioinformatics integrates biology, statistics, and computer science to develop and apply theory, methods, and tools for the collection, storage, and analysis of biological and related data. Bioinformatics plays a critical role in Read More...

This lesson provides an introduction to R in the context of single cell RNA-Seq analysis with Seurat. Learning Objectives Learn about options for analyzing your scRNA-Seq data. Learn about resources for learning R programming. Learn Read More...

Learning Objectives This tutorial was designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples. Throughout this tutorial we Read More...

1. Introduction and Learning Objectives This tutorial has been designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples that Read More...

The Advanced Biomedical Computational Science (ABCS) group focuses on applications of bioinformatics, computational and data science, and artificial intelligence to support NCI researchers. ABCS provides: • Subject matter expertise in genomics, proteomics, and imaging. • Machine learning/ Read More...

The geom functions require a mapping argument. The mapping argument includes the aes() function, which "describes how variables in the data are mapped to visual properties (aesthetics) of geoms" (ggplot2 R Documentation). If Read More...

Data visualization with ggplot2 Objectives To learn how to create publishable figures using the ggplot2 package in R. By the end of this lesson, learners should be able to create simple, pretty, and effective figures. Read More...

This lesson will introduce data wrangling with R. Attendees will learn to filter data using base R and tidyverse (dplyr) functionality. Learning Objectives Understand the concept of tidy data. Become familiar with the tidyverse packages. Read More...

Objectives Review important data wrangling functions Put our wrangling skills to use on a realistic RNA-Seq data set Data Wrangling Review Important functions by topic Importing / Exporting Data Importing and exporting data into the R Read More...

Practice Lesson 2 For the help sessions, we will work on processing sequences generated in Zhang Z, Feng Q, Li M, Li Z, Xu Q, Pan X, Chen W. Age-Related Cancer-Associated Microbiota Potentially Promotes Oral Squamous Read More...

Lesson 5: Microbial diversity, alpha rarefaction, alpha diversity Learning Objectives Understand the difference between alpha and beta diversity Introduce several alpha diversity metrics Understand what rarefaction is and why it is important Introduce the debate regarding Read More...

Lesson 2: Getting Started with QIIME2 Lesson Objectives Obtain sequence data and sample metadata Import data and metadata Discuss other useful QIIME2 features including view QIIME2, provenance tracking, and the QIIME2 forum. DNAnexus DNAnexus provides a Read More...

Introduction to ggplot2 Objectives Learn the ggplot2 syntax. Build a ggplot2 general template. By the end of the course, students should be able to create simple, pretty, and effective figures. Data Visualization in the tidyverse Read More...

Objectives To explore Bioconductor, a repository for R packages related to biological data analysis. To better understand S4 objects as they relate to the Bioconductor core infrastructure. To learn more about a popular Bioconductor S4 Read More...

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empanada v1.2 now released empanada-napari now comes with: MitoNet v1, NucleoNet v0.1 and DropNet v0.1(and other new useful modules) Empanada (EM PANoptic Any Dimension Annotation) is a tool Read More...