Search Results for: Tissue Embedding/Block Preparation (paraffin

What is Visium FFPE v2 with CytAssist? Visium FFPE v2 is sequencing-based spatial profiling technology developed by 10x Genomics. This assay can take mouse or human tissue sections on normal glass slides as input and Read More...

Employing spatial biology techniques enables acquisition of transcript and protein data from intact tissue sections, and in turn, spatial distribution information and cellular interaction patterns are revealed.

The NCI Clinical Research Correlatives Core provides non-CLIA-certified spectral flow cytometric assays to support clinical trials conducted in the CCR. The core specializes in immunophenotyping and immune monitoring assays. Established Technologies Spectral flow cytometry (Cytek), Read More...

What is Xenium? Xenium is a high-resolution, imaging-based in situ spatial profiling technology from 10x Genomics that allows for simultaneous expression analysis of RNA targets (currently in range of 100’s) within the same tissue section. Read More...

.row { display: flex; justify-content: space-around; align-items: flex-start; margin: 20px; } .column { text-align: center; padding: 10px; width: 30%; } .column img { display: block; margin: 0 auto; width: 150px; height: 150px; } .column strong { display: block; margin-top: 10px; } Background: Intravital microscopy (IVM) Read More...

CLIA-Certified Technologies Offered: Fragment Analysis for Micro-satellite Instability Detection, Pharmacoscan Array for Pharmacogenomics, Mutation Detection for PCR and Sanger Sequencing, DNA extraction from whole blood, saliva, FFPE tissues, buccal swabs, nails, hair, PBMCs, buffy coats, Read More...

CREx News & Updates August 2021 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Collaborative Research Exchange (CREx) News Site Spotlight FACILITY HIGHLIGHTS Learn more about services from the NHLBI Read More...

The NIH Center for Human Immunology, Inflammation, and Autoimmunity (CHI) is a trans-NIH resource whose mission is to provide a collaborative hub of advanced translational immunology for NIH clinical and pre-clinical studies. This uniquely structured Read More...

The Cancer and Inflammation Program – Microbiome and Genetics Core (CIP-MGC) grew out of the former CIP Genetics Core to meet the increasing need for sequencing and analysis of commensal microbiota within CIP and NCI. The Read More...

The CCR Building 41 Flow Cytometry Core is a full-service facility within the Center for Cancer Research that supports over 150 users representing 26 laboratories. The Core Facility provides instrument and software training, technical expertise, assay development, and Read More...

The Clinical Flow Cytometry Laboratory provides extensive support for NCI clinical protocols by providing diagnostic testing for leukemia and lymphoma in patients either on NCI clinical protocols or undergoing testing to determine eligibility for NCI Read More...

The facilities at AIM are available for use by the entire NIH intramural research community. While we welcome users with any size imaging project, AIM specializes in large, yearlong (or longer), collaborative research efforts with Read More...

The Mass Cytometry Core (MCC) provides access to cutting-edge platforms and expert technical support for high-dimensional single-cell analysis and spatial proteomic profiling. A central expertise of the core is Imaging Mass Cytometry (IMC), which allows Read More...

CREx News & Updates March 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight NIA Nonhuman Primate Core The NIA Nonhuman Primate Core supports multi-disciplinary translational aging projects for Read More...

CREx News & Updates February 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight ORS Division of Veterinary Resources (DVR) DVR is the central NIH lab animal support program Read More...

The Biomedical Engineering and Physical Science (BEPS) shared resource supports NIH’s intramural basic and clinical scientists on applications of engineering, physics, imaging, measurement, and analysis. BEPS is centrally located on the main NIH campus Read More...

The CCR Genomics Core is located in Building 41 on the NIH Bethesda campus. The primary goal of the Core is to provide investigators from CCR/NCI and other NIH Institutes access to genomic technologies and Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Intramural CryoEM (NICE) Consortium NICE provides NCI, NIAID, NIEHS, NICHD, NIDCR, NEI, and NIA investigators with Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Extramural Common Fund Resources Metabolomics Workbench Developed by the NIH Metabolomics Common Fund's National Metabolomics Data Repository ( Read More...

The Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Established Technologies Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), Intracellular markers, including cytokines and Read More...

The OSTR offers cutting-edge technology platforms to the CCR scientific community through centralized facilities. The videos accessed through this page are designed to introduce the various scientific methodologies OSTR makes available through the cores on Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Technology Event Biophysical Methods for Protein Interactions Monday, May 15 – Friday, May 19, 2023 This workshop will review the strategies of Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Cores on CREx NIBIB BEPS Micro Analytical Immunochemistry Unit The Micro Analytical Immunochemistry Unit employs a variety Read More...

The NIH Clinical Center Pharmacy Department provides pharmaceutical care to inpatients and outpatients on NIH intramural research protocols at the NIH Clinical Center. The Clinical Center facility encompasses 200 inpatient beds, 93 day-hospital stations, and 15 clinics. Clinical Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New CREx User Survey The CREx Team is carrying out a CREx User Survey. If you haven’t Read More...

Back Services: Biophysics Facility offers MST as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument reservation calendar.  Training includes the KD determination of a Read More...

NIH Intramural CryoEM Consortium (NICE) serves intramural investigators in all NIH IC’s. NICE provides access to state-of-the-art Titan Krios cryo-electron microscopes for atomic-resolution structure determination of protein, macromolecular complexes, membrane receptors, cellular organelles, and Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New Resources on CREx NIH NeuroBioBank (NBB) The NBB provides researchers with access to human post-mortem brain tissue Read More...

The Optical Microscopy Analysis Core (OMAC), formerly known as the Optical Microscopy Analysis Lab (OMAL),  focuses its research and development activities to quantitatively understand the molecular basis of three-dimensional (3D) cell organization, motility, invasion, and Read More...

The Staff Scientists/Clinicians (SSSC) Technical Enrichment Program (STEP) was established to provide SSSC’s an opportunity to compete for funding to gain comprehensive training in state-of-the-art techniques available through CCR Cores and Facilities. The Read More...

The National Cancer Institute (NCI) is developing a national repository of Patient-Derived Models (PDMs) comprised of patient-derived xenografts (PDXs), patient-derived organoids (PDOrg), and in vitro patient-derived tumor cell cultures (PDCs) and cancer-associated fibroblasts (CAFs). These Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More. New Resources on CREx NIMH Human Brain Collection Core (HBCC) The HBCC obtains human brain tissue, hair, and blood Read More...

[embed]https://youtu.be/yTl1Q0D7aZ0[/embed] 10x Genomics' supports multiple cancer research areas from single nucleotide and structural variant detection to single cell whole transcriptome gene expression analysis of thousands of individual Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Research Festival The NIH Research Festival highlights the groundbreaking science and the vibrant NIH community driving our Read More...

The Chemistry and Synthesis Center (CSC) of the National Heart, Lung, and Blood Institute (NHLBI) provides IRP scientists with targeted imaging probes and chemical tools that help accelerate cell-based assays, in vivo imaging studies, and Read More...

The centrally funded Statistics team within the Advanced Biomedical Computational Science group at the Frederick National Lab provides statistical consultation and data analysis support for NCI laboratories. We have broad-range expertise in biomedically relevant areas Read More...

The Division of Veterinary Resources Animal Surgery has supported large animal research and clinical care for more than 40 years. DVR surgeons provide outstanding and highly specialized large animal (rabbits, ungulates, etc.) research support in a Read More...

Many established and emerging technologies are available to CCR scientists.  This technology-rich environment makes the CCR a unique place to conduct scientific research.  Through the OSTR, the CCR continues to find Read More...

The COP evaluates novel therapies in pet dogs with cancer to improve outcomes for human patients and established the Comparative Oncology Trial Consortium (COTC), a collaborative effort of NCI and extramural comparative oncology centers at 24 Read More...

The NHLBI Transgenic Core’s main mission is to keep up with the latest advancements in genome engineering technologies and to provide state-of-the-art services to assist NIH scientists in generating genetically engineered animal models. In Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New Resources on CREx Interested in imaging large scale biological samples?  Volume electron microscopy allows for Read More...

[tabby title="Home"] About NICE-NIH Intramural CryoEM Consortium  NIH Intramural CryoEM Consortium (NICE) serves intramural investigators in all NIH IC’s. NICE provides access to state-of-the-art Titan Krios cryo-electron microscopes for atomic-resolution structure determination of Read More...

Back Services: Biophysics Facility offers MP as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument training calendar.  Training includes mass distribution analysis of a Read More...

The NCI High-Throughput Imaging Facility (HiTIF) works in a collaborative fashion with NCI/NIH Investigators by providing them with the necessary expertise, instrumentation, and software to develop and execute advanced High-Throughput Imaging (HTI) assays. These Read More...

Mass Spectrometry Section of the Collaborative Protein Technology Resource (Bldg. 37) Core Capabilities: Identification of proteins in complexes, organelles, subcellular fractions, or fluids. Global relative protein quantitation. Quantitation by isotopic labeling of cells in culture (SILAC) Read More...

The NIH Metabolomics Consortium (NIH-MC) is a shared research resource that performs metabolomics analysis (and related small molecule research) for investigators at all Institutes and Centers across the NIH Intramural Research Program. The NIH-MC is Read More...

The primary objective of the Protein Technology Core (PTC) is to establish an experimental screening pipeline for single-domain antibodies termed nanobodies. Nanobodies are antibody fragments consisting of a single monomeric variable antibody domain. The PTC, Read More...

The Magnetoencephalography (MEG) Core Facility specializes in MEG, a non-invasive procedure similar to electroencephalography (EEG), and aims to support research to better understand of the functioning of the brain. Established Technologies Magnetoencephalography (MEG) is a Read More...

Our operational objectives are to provide state-of-the-art OMICS technologies in support of the Genetics Branch (GB) investigators and collaborators. Research Services Wet Lab Single cell isolation from fresh, frozen, and FFPE tissue, DNA/RNA extractions Read More...

The Mouse Imaging Facility (MIF) is a shared, trans-NIH intramural resource for animal imaging studies. MIF provides access to state-of-the-art radiological imaging methods optimized for mice, rats, other animals, and tissue samples. MIF provides intellectual, Read More...

CREx News & Updates May 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Click below to learn how easy it is to navigate the CREx platform. These short videos will Read More...

The Division of Veterinary Resources’ (DVR) mission is to contribute to the advancement of NIH biomedical research programs by providing excellence in animal care, customer service, and specialized research services to support intramural research efforts. Read More...

Back Services: Biophysics Facility offers DLS as an open-access instrument. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Training includes DLS analysis of small- and large-molecular size Read More...

The Antibody Characterization Laboratory (ACL) is the laboratory responsible for the development of well-characterized monoclonal antibody reagents. The NCI’s Office of Cancer Clinical Proteomics Research funds ACL as a resource to the entire cancer Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New NIH Resource Resources Advance your research with the NIH Mouse Imaging Facility (MIF) The NIH Mouse Imaging Read More...

The function of the SAIP is to collaborate with NCI investigators in the development of mouse models, new molecular imaging probes for early detection and therapy, monitor tumors in vivo, and perform drug efficacy studies Read More...

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NISC’s role within NHGRI, and more broadly across NIH, aims to advance genome sequencing and its many applications, with a goal not simply to produce sequence data, but to produce the infrastructure required to Read More...

CREx News & Updates August 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight NCATS Functional Genomics Laboratory (FGL) FGL is designed to help NIH Investigators use the latest Read More...

The Spatial Imaging Technology Resource (formerly the Nanoscale Protein Analysis Section of the Collaborative Protein Technology Resource or CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery Read More...

Back Services: We offer a limited sample processing service using standard SEC-MALS and FFF protocols.  This service is intended for the occasional users of this system.  Researchers who expect to use this instrument Read More...

Back Services: We offer a limited sample processing service using standard SEC-MALS and FFF protocols.  This service is intended for the occasional users of this system.  Researchers who expect to use this instrument Read More...

Protein and Metabolite Characterization Core (PMCC), formerly known as the Protein Characterization Lab (PCL),  offers various technologies to CCR investigators to characterize proteins and metabolites. The core develops and applies state-of-the-art analytical technologies, primarily mass Read More...

In order to meet increasing demands from both NIH intramural and extramural communities for access to a small angle X-ray scattering (SAXS) resource, the Center for Cancer Research (CCR) under the leadership of Drs. Jeffrey Read More...

The Biopharmaceutical Development Program (BDP) provides resources for the development of investigational biological agents. The BDP supports feasibility through development and Phase I/II cGMP manufacturing plus regulatory documentation. The BDP was established in 1993. We Read More...

06/25/2024 - Register for the June Emerging Technologies Seminar to hear from Dr. Dana Pe’er of the Memorial Sloan Kettering Cancer Center. She will describe new bioinformatics tools for exploring the complex tumor Read More...

05/05/2025 - Insights from Scaled-up Cancer Tissue Proteomics

Ignoring these simple guidelines will greatly increase the chances that your data will be unanalysable and/or your experiment unpublishable. Prepare all samples at the same time or as close as possible. The same person Read More...

06/17/2025 - Due to the growing popularity of high-plex patial proteomic and tran criptomic technique , many analy i platform have been propo ed to make en e of the re ulting data. Nonethele , mo t platform Read More...

Users can run Unix commands within a Python Jupyter Notebook. To do this start a code block with "!" followed by the Unix command. For instance, use the pwd command in the code block Read More...

12/08/2022 - This webinar will introduce best practices for sharing NIH-funded data in generalist repositories including tips and guidance for generating high quality metadata to describe your data, preparing data files and documentation for sharing, considerations Read More...

Illumina Sequencing Technology Video Introduction to Illumina Sequencing Summary of Illumina DNA-seq applications and library preparation methods Summary of Illumina RNA-seq applications and library preparation methods

High resolution single cell profiling assays have provided an unprecedented view of many biological systems and processes, but the spatial context in which this biology is occurring is often crucial. Spatial profiling, including spatial transcriptomic Read More...

11/01/2022 - Join us to learn how Visium Spatial and Xenium In Situ platforms from 10x Genomics can help you push the boundaries of your research. Uncover molecular insights, dissect cell-type differences, investigate the adaptive immune Read More...

04/23/2024 - Join Dr. Kai Tan of the Children’s Hospital of Philadelphia as he discusses his cutting-edge technology, CytoCommunity, for the supervised and unsupervised discovery of tissue cellular neighborhoods. CytoCommunity offers a powerful Read More...

07/24/2025 - The "Data Vi ualization in R" erie focu e on u ing ggplot and the broader tidyver e eco y tem to create in ightful and cu tomizable vi ualization . It cover Read More...

07/23/2025 - This presentation will explore the next emerging stage where biobanking is characterised by being part of system - Biobanking 4.0. Biobanking has always been about data generation with tissue specimens providing the biological and genomic Read More...

12/07/2023 - This webinar will discuss and demonstrate experimental design considerations in variant analysis, including the origins of tissue samples (germline vs. somatic), whole exome (WES) or whole-genome sequencing (WGS), sample sizes and statistical power, quality Read More...

Genomic variations are typically categorized into different classes and are often denoted with a shortened acronym: SNP, a single nucleotide polymorphism - A change of a single base. INDEL, an insertion or a deletion - Read More...

The genes that exhibit higher expression in the tumor tissue (ie. log2 fold change ≥ 1 and false discovery rate ≤ 0.05) have been subsetted into the file hcc1395_deg_chr22_up_genes.txt .

Purpose of RNA sequencing and what biological questions can RNA sequencing answer Experimental considerations Sample preparation Replicates Technical noise Read depth More depth for low expression genes More depth for low expression differences between samples Read More...

A typical RNASEQ experiment involves several steps, only one of which falls within the realm of bioinformatics. Namely the Data Analysis step. Experimental Design What question am I asking How should I do it (does Read More...

Biostar Class - Sequencing Instruments Below are a list of links and resources mentioned in the BIOSTAR Sequencing Instruments class given on 06/10/20 and 06/11/20 Sequencing Technologies - Company Web Sites Illumina PacBio Oxford Nanopore 10X Genomics Read More...

06/01/2023 - CellTypist was first developed as a platform for exploring tissue adaptation of cell types using scRNA-seq semi-automatic annotations. Now it's an open source tool for automated cell type annotations as well as a  Read More...

03/15/2023 - This virtual hands-on workshop explores the fundamentals of machine learning using MATLAB. The participants will be introduced to machine learning techniques to quickly explore data, use classification and regression apps to interactively train, compare Read More...

10/13/2022 - This virtual hands-on workshop explores the fundamentals of machine learning using MATLAB. The participants will be introduced to machine learning techniques to quickly explore data, use classification and regression apps to interactively train, compare Read More...

05/04/2021 - Register Session Description This virtual hands-on workshop explores the fundamentals of machine learning using MATLAB. The participants will be introduced to machine learning techniques to quickly explore data, use classification and regression apps to Read More...

04/08/2021 - https://nih-irp-singlecell.github.io/SC-UsersGroup/ Presenter: George Emanuel PhD, Co-founder Vizgen, Director of Technology and Partnerships. Abstract: Biological systems are comprised of numerous cell types, intricately organized to form functional tissues and organs. Cell Read More...

07/31/2025 - NIDDK Biostats Seminar Series: From Research Study Design to Collecting, Managing, and Analyzing Data. Learning Objectives: Be able to identify, load, and use R resources/packages based upon needs and experience level with R. 1. Read More...

07/09/2025 - From image analy i to federated learning and multimodal modeling, generative AI can be u ed to tudy ob ervational ucce ion acro cale and data type . At DCEG' Data cience and Engineering Re Read More...

06/26/2025 - Join u for a webinar introducing MIRA, a Python oftware package de igned for the analy i of ingle-cell RNA- eq, ATAC- eq, and multimodal data. Di cover how MIRA help decode the ci Read More...

03/26/2025 - Developing artificial intelligence (AI) schemes to assist the clinician towards enabling precision medicine approaches requires development of objective markers that are predictive of disease response to treatment or prognostic of longer-term patient survival. The Read More...

03/19/2025 - This one hour and a half online training provides an accessible introduction to artificial intelligence (AI) using MATLAB. Designed for beginners, the session covers fundamental concepts in AI and machine learning, introduces intuitive tools Read More...

02/18/2025 - This class will introduce basic experimental and analytical considerations for bulk RNA sequencing, a technique that is used to profile gene expression at the tissue level. Information taught serves as the foundation for participants Read More...

07/17/2024 - Dear Colleagues,   Please join us on Wednesday, July 17, 2024, when Dr. Olivier Gevaert from Stanford University will discuss leveraging data at different scales for personalized diagnosis, prognosis, and therapy in the fields of Read More...

Clustering is used to group cells by similar transcriptomic profiles. Seurat uses a graph based clustering method. You can read more about it here . The first step is to compute the nearest neighbors of each Read More...

In this tutorial, we are using data from Nanduri et al. 2022, Epigenetic regulation of white adipose tissue plasticity and energy metabolism by nucleosome binding HMGN proteins , published in Nature Communications . The raw count matrices are Read More...

05/23/2024 - An exciting opportunity at the intersection of the biomedical sciences and machine learning stems from the growing availability of large-scale multi-modal data (imaging-based and sequencing-based, observational and perturbational, at the single-cell level, tissue-level, and Read More...

Check for primers Generate an ASV count table and representative sequence file Understand the difference between OTU picking and denoising The two primary files that will be used throughout any microbiome analysis are the feature Read More...

Purpose of RNA sequencing and what biological questions can RNA sequencing answer Experimental considerations Sample preparation Replicates Technical noise Read depth More depth for low expression genes More depth for low fold differences Read length Read More...

Purpose of RNA sequencing and what biological questions can RNA sequencing answer Experimental considerations Sample preparation Replicates Technical noise Read depth More depth for low expression genes More depth for low fold differences Read length Read More...

This tool was created and is maintained by the Laboratory of Human Retrovirology and Immunoinformatics at Frederick National Lab. DAVID is used for functional analysis. Given an input gene list, DAVID will inform us of Read More...

This tool was created and is maintained by the Laboratory of Human Retrovirology and Immunoinformatics at Frederick National Lab. DAVID is used for functional analysis. Given an input gene list, DAVID will inform us of Read More...

Overview of wet lab procedures Library preparation process, including Adapters and indices Single and paired end sequencing Strandedness Coverage and depth Spike-ins Replicates Batch effects Overview of analysis procedures References and annotation files needed for Read More...

Provides a gene-centric view which lists the genes and their associated annotation terms... -- DAVID help documents See https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717906/ for difference between pathway and network analysis DAVID Read More...

Generating the Data General Rules for Sample Preparation Ignoring these simple guidelines will greatly increase the chances that your data will be unanalysable and/or your experiment unpublishable. Prepare all samples at the same time Read More...

05/31/2023 - Please join us on May 31 when Harvard University’s John Quackenbush, Ph.D., will present “ Why Networks Matter: Embracing Biological Complexity. ” Dr. Quackenbush will share multiple examples illustrating the importance of network models. He Read More...

11/10/2022 - Learn how to include generalist repositories in data sharing plans as part of your preparation for the new NIH Data Management and Sharing Policy beginning in January 2023. This webinar will include guidance on selecting Read More...

10/19/2022 - Speaker: Ron Germain, M.D., Ph.D. NIH Distinguished Investigator NIAID, NIH Dr. Germain received his Sc.B. and Sc.M. from Brown University in 1970 and his M.D. and Ph.D. from Harvard Read More...

04/13/2022 - The first and the most common single cell application is single cell RNA-Seq, which enables scientists to focus on the gene expression profiles of individual cells rather than on tissue averages. Knowledge of expression Read More...

05/17/2021 - For our next meeting we will be having a guest lecture by Dr. Josh Waterfall from Institut Curie. Abstract: Non small cell lung cancers (NSCLC) are known to be recurrently infiltrated by multiple immune Read More...

04/12/2021 - Tumor heterogeneity and plasticity provide a driving force for tumor progression and metastasis as well as treatment response. Tumor heterogeneity can be explained by the same mechanisms that govern the developmental programs of an Read More...

04/16/2026 - The ability to measure gene expression levels for individual cells (vs. pools of cells) and with spatial resolution is crucial to address many important biological and medical questions, such as the study of stem Read More...

03/27/2025 - Please note: Registration is required to get the Meeting Link for this event. Please pre-register. Aviv Regev is head of Genentech Research and Early Development. Formerly, Regev was Chair of the Faculty and Core Read More...

Long read sequencing was recently named 2022’s method of the year by Nature Methods . Long read sequencing technologies, those that generate sequence reads with lengths of 10s of kilobases or longer have several advantages over Read More...

01/21/2025 - About the Speaker: Hari completed his PhD in Cell Biology at the University of Toronto, where he used single-molecule imaging techniques to reveal how multimolecular interactions govern early signaling events in individual immune cells. Read More...

11/06/2024 - Join Our Training on Spatial Omics Data Analysis with MAWA (Multiplex Analysis Web Apps).   Want to learn how to process and analyze your spatial proteomics/transcriptomics data? Join us for four virtual hour-long sessions Read More...

10/29/2024 - Join Our Training on Spatial Omics Data Analysis with MAWA (Multiplex Analysis Web Apps).   Want to learn how to process and analyze your spatial proteomics/transcriptomics data? Join us for four virtual hour-long sessions Read More...

10/22/2024 - Join Our Training on Spatial Omics Data Analysis with MAWA (Multiplex Analysis Web Apps).   Want to learn how to process and analyze your spatial proteomics/transcriptomics data? Join us for four virtual hour-long sessions Read More...

10/15/2024 - Join Our Training on Spatial Omics Data Analysis with MAWA (Multiplex Analysis Web Apps).   Want to learn how to process and analyze your spatial proteomics/transcriptomics data? Join us for four virtual hour-long sessions Read More...

06/13/2024 - The schedule for this week consists of one presentation: Lorenz Adlung, UMC Hamburg-Eppendorf, will discuss: scMod: Marrying machine learning and deterministic modelling of longitudinal single-cell data Single-cell-based methods such as flow cytometry or single-cell Read More...

In this tutorial, we will continue to use data from Nanduri et al. 2022, Epigenetic regulation of white adipose tissue plasticity and energy metabolism by nucleosome binding HMGN proteins , published in Nature Communications . As a reminder, Read More...

In this tutorial, we will continue to use data from Nanduri et al. 2022, Epigenetic regulation of white adipose tissue plasticity and energy metabolism by nucleosome binding HMGN proteins , published in Nature Communications . As a reminder, Read More...

The Advanced Biomedical Computational Science (ABCS) group focuses on applications of bioinformatics, computational and data science, and artificial intelligence to support NCI researchers. ABCS provides: • Subject matter expertise in genomics, proteomics, and imaging. • Machine learning/ Read More...

01/03/2024 - on Wednesday, January 3rd at noon in Building 41, Conference Room C507/C509 and online. In-person attendance is encouraged.  Dr. Larson's research is focused on understanding gene expression in eukaryotic Read More...

Lesson 12: RNA sequencing review 1 Learning objectives Here, we will do a quick review of what we have learned about RNA sequencing in Lessons 8 through 11. Accessing the Biostar handbook The URL for the Biostar handbook is Read More...

Lesson 17: RNA sequencing review 2 Learning objectives This lesson will serve as comprehensive review of Module 2. We will spend roughly the first hour reviewing the Module 2 material the second hour answering specific questions from the poll Read More...

Database for Annotation, Visualization and Integrated Discovery (DAVID) - practicing what we learned Learning objectives In this practice session, we will practice using DAVID. Practicing what we learned To help us apply what we learned Read More...

Database for Annotation, Visualization and Integrated Discovery (DAVID) - an overview Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 17 review In the previous class, we got an overview of Read More...

Bioinformatics for beginners Module 2: Introduction to RNA sequencing In this module, we will use the Human Brain Reference and Universal Human Reference RNA sequencing datasets to learn about RNA sequencing. Each lesson will be followed Read More...

This page uses context taken directly from the Biostar Handbook by Istvan Albert. Remember to activate the class bioinformatics environment. conda activate bioinfo Introduction to Genomic Variation Genomic variations are typically categorized into different classes Read More...

Let's use the tool Trimmomatic to clean up the adapters and the poor quality reads for SRR1553606. For help with Trimmomatic type trimmomatic --help at the command line. Before getting started with using trimmomatic, Read More...

Let's now take a look at our final differential analysis results table (results_with_gene_names_labeled.txt), using the SLC2A11 gene as an example and below we use the column command to Read More...

Managing Bioinformatics Projects with Jupyter Lab Learning Objectives After this class, participants will have obtained the foundation needed to start using Jupyter Lab as an all-in-one place to maintain code, output, and other description of Read More...

The identification of cell types in single cell genomics data (cell type annotation) can provide new insights into cellular identities, heterogeneity, and functional roles. Cell type annotation is challenging Read More...

02/17/2023 - Dr. Brendan Miller is a post-doctoral research fellow at Johns Hopkins University in the Department of Biomedical Engineering. On Friday Feb 17, 1:00-2:00 PM, he will be discussing some tools he has recently helped develop Read More...

10/04/2022 - Speaker: Steven D Cappell NIH Stadtman Investigator Center for Cancer Research – National Cancer Institute – Bethesda MD My laboratory is integrating quantitative image analysis with genetic, biochemical, and systems biology approaches to understand how cells Read More...

08/10/2022 - For our next CDSL webinar we will have two fellows’ talks from Dr. Zisha Zhong and Arati Rajeevan . Zisha’s talk details: Title:  Multi-task Feature Learning to train Interpretable Encoders on Histopathology Images Digital Read More...

06/06/2022 - Join the June NCI Imaging and Informatics Community Webinar for a discussion on the recent contributions from Dr. Mirabela Rusu’s Personalized Integrative Medicine Laboratory (PIMed) at Stanford University. Recent laboratory contributions include: registering Read More...

05/19/2022 - THIS EVENT HAS BEEN CANCELLED Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma of childhood and are subdivided into three major histomorphologic subtypes: alveolar (ARMS), embryonal (ERMS), or spindle/sclerosing (SSRMS).  Patients with Read More...

10/13/2021 - Register for the  2021 Clinical Proteomics Tumor Analysis Consortium (CPTAC) Virtual Scientific Symposium  to hear CPTAC investigators share their latest discoveries in the field of cancer proteogenomics, cancer research, and  data analysis  tools. In addition Read More...

08/05/2021 - Understanding the biology in transcription factor binding site data requires a comprehensive and integrated approach and must be supplemented with highly-curated data content from multiple sources. Genomatix is a computational biology company with a 22 Read More...

06/17/2021 - The intrinsic stochasticity of transcription leads to gene expression variation across cells in a clonal cell population. The expression variation can translate into phenotypic variation that can persist through several rounds of cell division. Read More...

06/09/2021 - The CCR Office of Science and Technology Resources (OSTR) is pleased to host a virtual technology seminar given by Advanced Cell Diagnostics (ACD) and NCI Cores at FNLCR. Presenters: Jyoti Phatak, MS | Advanced Cell Read More...

06/07/2021 - A multidisciplinary network of researchers and clinicians dedicated to improving early detection, diagnosis, prognosis and treatment of liver cancer. Presenter: Shalev Itzkovitz, PhD Associate Professor Department of Molecular Cell Biology Weizmann Institute of Science Read More...

05/19/2021 - Register Description: IPA’s Analysis Match Explorer was launched in March 2020 and this upgrade is being evaluated for purchase by NCI. NCI scientists will have no-charge access to Analysis Match Explorer for a limited Read More...

05/03/2021 - Abstract: We will discuss technical advantages of a personalized and tumor-informed multiplex PCR next generation sequencing assay, called Signatera™, that enables a sensitive, specific, and dynamic detection of molecular disease burden in cell-free DNA ( Read More...

04/20/2021 - Abstract: Single-cell genome-wide profiling offers an approach to map transitional cell states during cell differentiation, disease onset, and drug response. Lineage-tracing, in which cells are labeled with hereditary markers, offers an approach to establishing Read More...

03/29/2021 - For coming Monday's CDSl webinar, we'll be hosting Dr. Yun Liu from Google Health. Abstract: This talk will briefly cover two categories of our work: deep learning to identify dermatology conditions from Read More...

03/05/2021 - Overview: The Human Cell Atlas (HCA) is an ambitious global initiative that aims to create a comprehensive reference map of all human cells—the fundamental units of life—as a basis for both understanding Read More...

02/08/2021 - Presenter: Dr. Marjan Gucek, director for the NHLBI Proteomic Core Summary: “Proteomics Core provides access to mass spectrometry and gel based proteomics for identification and quantitation of proteins and their post-translational modifications (PTM). Our Read More...

Learning Objectives This tutorial was designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples. Throughout this tutorial we Read More...

10/24/2024 - NIH Text Mining and Natural Language Processing SIG is pleased to welcome you to this special event featuring two extraordinary speakers focused on the applications of Deep Learning in Computational Biology.  & Read More...

This lesson provides an introduction to R in the context of single cell RNA-Seq analysis with Seurat. Learning Objectives Learn about options for analyzing your scRNA-Seq data. Learn about resources for learning R programming. Learn Read More...

1. Introduction and Learning Objectives This tutorial has been designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples that Read More...

Lesson 3: Creating a feature table Lesson Objectives Check for primers Generate an ASV count table and representative sequence file Understand the difference between OTU picking and denoising The two primary files that will be used Read More...

RNA-SEQ Overview What is RNASEQ ? RNA-Seq (RNA sequencing), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment. (Wikipedia) Strictly speaking this could be any Read More...

We will build a database out of all features of the 2014 Ebola genome under accession number KM233118. This data will go into a new directory named "db_2014". mkdir -p db_2014 # Get the 2014 Ebola Read More...

In lesson 9, we learned that reference genomes came in the form of FASTA files, which essentially store nucleotide sequences. In this lesson, we will learn about the FASTQ file, which is the file format that Read More...

Lesson 10: Introducing the FASTQ file and assessing sequencing data quality Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 9 Review In the previous lesson, we explored the reference genomes and Read More...

Lesson 11: Merging FASTQ quality reports and data cleanup Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 10 Review In the previous lesson, we learned about the structure of the FASTQ Read More...

Database for Annotation, Visualization and Integrated Discovery (DAVID) - an overview Lesson 17 review In the previous class, we got an overview of functional and pathway analysis, which help to put RNA sequencing results into biological Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Remember to activate the bioinformatics environment and create a directory for today's work. conda activate bioinfo mkdir blast cd blast What is Read More...

Lesson 16: RNA sequencing review and classification based analysis Before getting started, remember to be signed on to the DNAnexus GOLD environment. Review In the previous classes, we learned about the steps involved in RNA sequencing Read More...

04/03/2023 - Workshop Description:  The application of AI to cancer research holds promise to accelerate new discoveries, enable early detection, improve diagnosis, and spur development of new therapies for cancer. Machine learning and other forms of Read More...