Search Results for: differential interference contrast (Nomarsky) imaging

The Spatial Imaging Technology Resource (formerly the Nanoscale Protein Analysis Section of the Collaborative Protein Technology Resource or CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery Read More...

Back Services: Biophysics Facility offers MP as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument training calendar.  Training includes mass distribution analysis of a Read More...

Back Services: This instrument is not user accessible.  We provide both data collection and data analysis services. Location: Building 50, room 3331 Description: An analytical ultracentrifuge is equipped with absorption and interference optical systems that monitor Read More...

The Clinical Image Processing Service (CIPS) offers timely and accurate advanced image processing of diagnostic radiology images for clinical care, research, and training. CIPS’ functions include clinical services and scientific researches. Established Technologies CIPS can Read More...

The Biophysics Resource (BR) was established in January 2001. Our mission is to provide CCR investigators with access to both the latest instrumentation and expertise in characterizing the biophysical aspects of systems under structural investigation. The Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New NIH Resource Resources Advance your research with the NIH Mouse Imaging Facility (MIF) The NIH Mouse Imaging Read More...

The Biophysics Core’s mission is to provide support in the study of macromolecular interactions, dynamics, and stability by offering consultations, training, professional collaborations, and instrument access. General Services Multi-technique molecular interaction studies, Kinetic and Read More...

The Magnetoencephalography (MEG) Core Facility specializes in MEG, a non-invasive procedure similar to electroencephalography (EEG), and aims to support research to better understand of the functioning of the brain. Established Technologies Magnetoencephalography (MEG) is a Read More...

.row { display: flex; justify-content: space-around; align-items: flex-start; margin: 20px; } .column { text-align: center; padding: 10px; width: 30%; } .column img { display: block; margin: 0 auto; width: 150px; height: 150px; } .column strong { display: block; margin-top: 10px; } Background: Intravital microscopy (IVM) Read More...

The Behavioral Health Core (BHC) is comprised of the two separate but interrelated components: Psychosocial Support and Research Program, and, Health Psychology and Neurobehavioral Research Program., These teams provide specialized expertise, such as patient-reported and Read More...

The NHLBI Murine Phenotyping Core carries out physiologic and behavioral testing in a diversity of mouse models for NHLBI and other NIH institutes.Established TechnologiesCardiovascular Phenotyping, Metabolic Phenotyping, Pulmonary Phenotyping, Behavioral Phenotyping, Exercise Physiology, Echocardiography, Read More...

Back Services: Biophysics Facility offers Octet as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument reservation calendar.  Training includes a full analysis of a Read More...

Employing spatial biology techniques enables acquisition of transcript and protein data from intact tissue sections, and in turn, spatial distribution information and cellular interaction patterns are revealed.

The CCR Microscopy Core provides NCI investigators access to state-of-the-art imaging tools and techniques, including light sheet fluorescence, high-resolution confocal, multi-photon, and super-resolution microscopy. The mission of the CCR Microscopy Core Facility is to support Read More...

The NIH MRI Research Facility (NMRF) is a shared, intramural resource for human imaging studies. The NMRF is supported by all Institutes at NIH and reports to the Shared Resources Subcommittee (SRS) of the Scientific Read More...

As a multi-user facility, the different instruments provide a wide range of imaging modes for EIB scientists, from standard immunohistochemistry, through brightfield and wide-field epifluorescence imaging, to highly complex live cell confocal microscopy and super-resolution Read More...

The Chemistry and Synthesis Center (CSC) of the National Heart, Lung, and Blood Institute (NHLBI) provides IRP scientists with targeted imaging probes and chemical tools that help accelerate cell-based assays, in vivo imaging studies, and Read More...

The Molecular Pathology Unit is intended to spearhead opportunities for bridging basic and clinical research efforts by more precisely optimizing the development, characterization, and utilization of models of human disease. The initiative approach includes both Read More...

The NIH Biowulf Cluster provides researchers with a world-class system to assist in solving complex biomedical problems as diverse as gene variation in worldwide human populations, deep learning to model protein structures, and PET brain Read More...

Home About the Biophysics Core Biophysics Core Services [tabby title="Instrumentation"] NHLBI Biophysics Core The Biophysics Core Facility: Overview Core Facilities provide scientific resources, cutting-edge technologies and novel approaches to support DIR scientists. Availability of Read More...

LCMB Microscopy Core offers live cell imaging technologies as well as super-resolution, fluorescence lifetime and confocal imaging systems for immunofluorescence. Our confocal instruments are a Leica SP8 laser scanning confocal microscope and a Nikon spinning Read More...

The core provides access to several different state-of-the-art 3D microscopes as well as computers to visualize and process image data. The facility houses equipment for 2D or 3D imaging of fixed and living specimens. High Read More...

The LCBG Microscopy Core offers imaging technologies and training. The Core has established instrumentation for for 2D and 3D imaging of both fixed and living specimens.

CCR VOLUME Electron Microscopy Core (CVEMC),  formerly known as volume Electron Microscopy (vEM), aims to develop new techniques in volume electron microscopy (vEM) and, in collaboration with CCR Investigators, apply these techniques to research cell Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New Resources on CREx Interested in imaging large scale biological samples?  Volume electron microscopy allows for Read More...

NCI established the Nanotechnology Characterization Laboratory (NCL) to support the extramural research community to accelerate the progress of nanomedicine by providing preclinical characterization and safety testing of nanoparticles. It is a collaborative effort between NCI, Read More...

Back Services: Biophysics Facility offers MST as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument reservation calendar.  Training includes the KD determination of a Read More...

Employing spatial biology techniques enables acquisition of transcript and protein data from intact tissue sections, and in turn, spatial distribution information and cellular interaction patterns are revealed.

The Biomedical Engineering and Physical Science (BEPS) shared resource supports NIH’s intramural basic and clinical scientists on applications of engineering, physics, imaging, measurement, and analysis. BEPS is centrally located on the main NIH campus Read More...

CREx News & Updates August 2021 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Collaborative Research Exchange (CREx) News Site Spotlight FACILITY HIGHLIGHTS Learn more about services from the NHLBI Read More...

The Electron Microscopy Core (EMC), formerly known as Electron Microscopy Lab (EML),  offers investigators access to unique expertise and EM technologies that allow CCR Investigators to explore new avenues of research in order to enhance Read More...

CREx News & Updates March 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight NIA Nonhuman Primate Core The NIA Nonhuman Primate Core supports multi-disciplinary translational aging projects for Read More...

CREx News & Updates November 2021 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight FACILITY HIGLIGHTS Learn more about services from the NINDS Quantitative Magnetic Resonance Imaging Core. NINDS Read More...

The Mass Cytometry Core (MCC) provides access to cutting-edge platforms and expert technical support for high-dimensional single-cell analysis and spatial proteomic profiling. A central expertise of the core is Imaging Mass Cytometry (IMC), which allows Read More...

October 6, 2022 crex.nih.gov CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Science & Technology Seminars and Training Events Upcoming Seminars and Educational Opportunities The following Read More...

NanoString Technology The nCounter® Analysis System is an automated, multi-application, digital detection and counting system which directly profiles up to 800 molecules simultaneously from a single sample using a novel barcoding technology. Profile hundreds of mRNAs, Read More...

Back Services: Biophysics Facility offers ZetaView as an open-access instrument. First-time users must complete a short training session before using it for the first time. Training includes instrument calibration and size analysis of a standard Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New CREx User Survey The CREx Team is carrying out a CREx User Survey. We would greatly appreciate Read More...

The OSTR offers cutting-edge technology platforms to the CCR scientific community through centralized facilities. The videos accessed through this page are designed to introduce the various scientific methodologies OSTR makes available through the cores on Read More...

CREx News & Updates July 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Click below to learn how easy it is to navigate the CREx platform. These short videos will Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New CREx User Survey The CREx Team is carrying out a CREx User Survey. If you haven’t Read More...

Back Services: We offer a limited sample processing service using standard SEC-MALS and FFF protocols.  This service is intended for the occasional users of this system.  Researchers who expect to use this instrument Read More...

Back Services: We offer a limited sample processing service using standard SEC-MALS and FFF protocols.  This service is intended for the occasional users of this system.  Researchers who expect to use this instrument Read More...

The PET Department, CC, functions as a core facility that supports basic, translational, and clinical research using PET. It is a vertically integrated facility, with resources to produce positron-emitting radionuclides, manufacture PET radiopharmaceuticals in a Read More...

The FNLCR Molecular Histopathology Laboratory (MHL) provides comprehensive veterinary pathology support for animal health monitoring, biomarker discovery and validation, drug development, genomics, and proteomics on a cost recovered basis. The MHL is organized into multiple Read More...

The CCR Animal Resource Program (ARP) plans, develops, and coordinates laboratory animal resources for CCR’s research programs. We also provide training, imaging, and technology development in support of moving basic discoveries to the clinic. Read More...

The Neurorehabilitation and Biomechanics Research Section (also referred to as the NAB LAB) is a multidisciplinary group of highly qualified scientists, clinical and technical staff, and trainees with diverse backgrounds including medical, physical therapy, neuroscience Read More...

Many established and emerging technologies are available to CCR scientists.  This technology-rich environment makes the CCR a unique place to conduct scientific research.  Through the OSTR, the CCR continues to find Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Cores on CREx NIBIB BEPS Micro Analytical Immunochemistry Unit The Micro Analytical Immunochemistry Unit employs a variety Read More...

CREx News & Updates June 2021 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Collaborative Research Exchange (CREx) News Site Spotlight FACILITY HIGHLIGHTS Visit here to learn about services from Read More...

The Pathology Service, Division of Veterinary Resources (DVR), Office of Research Services (ORS), provides diagnostic gross and histopathologic evaluation of any species of laboratory animals used in the NIH Intramural Research Program due to morbidity, Read More...

October 6, 2022 crex.nih.gov CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Science & Technology Seminars and Training Events Upcoming Seminars and Educational Opportunities The following Read More...

Proteomic Data Common (PDC) represents the NCI’s largest public repository of proteogenomic comprehensive tumor datasets, essentially a Proteogenomic Cancer Atlas. It was developed to advance our understanding of how proteins help shape the risk, Read More...

The NCI Cloud Resources are components of the NCI Cancer Research Data Commons (CRDC) that bring data and computational power together to enable cancer research and discovery. These cloud-based platforms eliminate the need for researchers Read More...

What is Xenium? Xenium is a high-resolution, imaging-based in situ spatial profiling technology from 10x Genomics that allows for simultaneous expression analysis of RNA targets (currently in range of 100’s) within the same tissue section. Read More...

The Laboratory Animal Sciences Program (LASP) of the Frederick National Laboratory operates a Gnotobiotics Facility (GF) to support research focused on the role of microbiota in cancer inflammation, pathogenesis, and treatment response. The GF can Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New NIH Resource Resources Derive Greater Insights and Accelerate your Research Using Bioinformatic Tools! CREx is an NIH Read More...

CREx News & Updates January 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight NCI ANTIBODY CHARACTERIZATION LABORATORY (ACL) The ACL specializes in rigorously validating antibodies for signaling and Read More...

The Division of Veterinary Resources Animal Surgery has supported large animal research and clinical care for more than 40 years. DVR surgeons provide outstanding and highly specialized large animal (rabbits, ungulates, etc.) research support in a Read More...

CREx News & Updates June 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Click below to learn how easy it is to navigate the CREx platform. These short videos will Read More...

CCR Office of Science and Technology Resources (OSTR) The Center for Cancer Research (CCR) Office of Science and Technology Resources (OSTR) explores and evaluates the latest technologic advancements and then makes them available to all Read More...

The Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Established Technologies Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), Intracellular markers, including cytokines and Read More...

[tabby title="Home"] About NICE-NIH Intramural CryoEM Consortium  NIH Intramural CryoEM Consortium (NICE) serves intramural investigators in all NIH IC’s. NICE provides access to state-of-the-art Titan Krios cryo-electron microscopes for atomic-resolution structure determination of Read More...

Our operational objectives are to provide state-of-the-art OMICS technologies in support of the Genetics Branch (GB) investigators and collaborators. Research Services Wet Lab Single cell isolation from fresh, frozen, and FFPE tissue, DNA/RNA extractions Read More...

The CCR Genomics Core is located in Building 41 on the NIH Bethesda campus. The primary goal of the Core is to provide investigators from CCR/NCI and other NIH Institutes access to genomic technologies and Read More...

05/20/2024 - Hybrid (in-person location in Rockville, MD) Virtual attending via  WebEx Meeting, link will be available two weeks prior to the meeting date . Attend the  2024 Co-Clinical Imaging Research Resource Program (CIRP) Read More...

03/26/2025 - Developing artificial intelligence (AI) schemes to assist the clinician towards enabling precision medicine approaches requires development of objective markers that are predictive of disease response to treatment or prognostic of longer-term patient survival. The Read More...

03/19/2025 - Clinical Molecular Imaging AI

04/23/2024 - Dr. Stephanie Harmon obtained her Ph.D. in medical physics at the University of Wisconsin–Madison and did her postgraduate training at Leidos Biomedical Research where she worked within the Molecular Imaging Read More...

04/22/2024 - Dear Colleagues,    In this webinar, you'll get an introduction to XNAT. XNAT is an open-source platform for managing, processing, and sharing medical imaging and related data in research settings. & Read More...

The primary means of running differential expression in Seurat is through the FindMarkers function. The main usage for this function is as follows: FindMarkers(object,ident.1= ..., ident.2=..., test.use="wilcox", min.pct = 0.01, logfc. Read More...

Differential Expression Differential expression involves the comparison of normalized expression counts of different samples and the application of statistical measures to identify quantitative changes in gene expression between the different samples. Normalization and Statistical Significance Read More...

01/15/2026 - Gil Kanfer, PhD, of the NCI CCR High-Throughput Imaging Facility (HiTIF), in the Laboratory of Receptor Biology and Gene Expression (LRBGE) , will present the spatial biology analysis stack HiTIF is building to support Center Read More...

11/05/2025 - This talk delves into the innovative utilization of generative AI in propelling biomedical research forward. By harnessing single-cell sequencing data, we developed scGPT, a foundational model that extracts biological insights from an extensive dataset Read More...

09/17/2025 - Examples of combining visualization and computation in brain imaging. 

05/22/2024 - This seminar provides an overview of differential expression testing with Seurat. Topics to be covered include preparing data for differential gene expression, d ifferential gene analysis between specific groups, d ifferential gene analysis for Read More...

05/29/2025 - Prostate cancer exhibits significant intratumoral heterogeneity, driving a spectrum of phenotypes ranging from indolent disease to aggressive metastasis, challenging risk stratification and treatment. A key feature of this heterogeneity is clonal diversity, encompassing both Read More...

Differential expression analysis is the process of identifying genes that have a significant difference in expression between two or more groups. For many sequencing experiments, regardless of methodology, differential analysis lays the foundation of the Read More...

03/11/2025 - In this class, participants will get hands-on experience with generating and interpreting differential gene expression analysis results from bulk RNA sequencing. In addition, participants will learn to generate common plots used to visualize bulk Read More...

03/06/2025 - This class introduces participants to the first steps to differential expression analysis in bulk RNA sequencing which involves filtering out noise from (ie. genes without expression across samples) and performing QC on the gene Read More...

One particular critique of differential expression in single cell RNASeq analysis is p-value "inflation," where the p-values get so small that there are far too many genes exist with p-values below 0.05, even after Read More...

1. Introduction and Learning Objectives This tutorial has been designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples that Read More...

01/22/2026 - Attend this webinar to learn more about XNAT Scout—a new extension of the XNAT imaging informatics platform that’s designed to close the gap between artificial intelligence (AI) model development and clinical deployment. Read More...

01/14/2026 - During this event, Dr. Emilie Roncali, an associate professor at UC Davis, is tackling an intersection of two critical data science concepts in cancer research: theranostics and digital twins. Dr. Roncali will give you Read More...

04/08/2025 - Dive into the specialized use of mixed-effects models tailored to address the challenges of inter-reader variability in medical imaging studies. This presentation delves into how these models adeptly manage the random effects introduced by Read More...

11/26/2024 - Dr. Daniel Orringer received his M.D. from The Ohio State University, completed his residency in neurological surgery at the University of Michigan Health System, and fellowship training in neuro-oncology at the Massachusetts General-Brigham Read More...

From the paper where this data was obtained, the following (incomplete) list of gene markers was obtained: Mmp3: preadipocytes Mki67: proliferating cells Fabp4: differentiating beige adipocytes and differentiated beige adipocytes Scd1: differentiated beige adipocytes Ucp1: Read More...

Regarding computational requirements, rule of thumb is to keep it at 8 samples or less when running on a laptop. You may be able to stretch it to 10 samples, but any more than that strains the Read More...

The role of cell clustering is to identify cells with similar transcriptomic profiles by computing Euclidean distances across genes . However, scRNA-Seq experiments include highly dimensional data, with each cell associated with the expression of thousands Read More...

In Seurat (since version 4), differential analysis requires a preprocessing step to appropriately scale the normalized SCTransform assay across samples: adp = PrepSCTFindMarkers(adp) Found 8 SCT models. Recorrecting SCT counts using minimum median counts: 8146 As covered earlier, Read More...

05/06/2024 - Have you been looking for ways to use artificial intelligence (AI) in clinical practice but not sure where to start? Attend this webinar for tips from Dr. Anant Madabhushi on applying AI in precision Read More...

05/19/2020 - UCSC Xena ( xena.ucscedu/) is a web-based visual integration and exploration tool for multi-omic data and associated clinical and phenotypic annotations. Xena showcases seminal cancer genomics datasets from TCGA and the Pan-Cancer Atlas, as Read More...

12/19/2016 - iPathwayGuide is a gene and protein expression analysis tool that uses a systems biology approach to identify significantly impacted pathways, gene ontology terms, diseases, and predicted microRNAs based on the given gene or protein Read More...

02/12/2026 - Proteins mediate the critical processes of life and beautifully solve the challenges faced during the evolution of modern organisms. Dr. Baker’s goal is to design a new generation of proteins that Read More...

01/16/2026 - Part of the Childhood Cancer Data Initiative (CCDI) Webinar Series During this webinar, experts from the USC Norris Comprehensive Cancer Center (NCCC) and Children's Hospital Los Angeles (CHLA) will present: An overview of Read More...

01/16/2026 - Speakers: James Amatruda, M.D., Ph.D. David Buckley, Ph.D. Jennifer Cotter, M.D. Alexander Markowitz, Ph.D. Moderator: Warren Kibbe, Ph.D., FACMI During this webinar, experts from the USC Norris Comprehensive Read More...

12/02/2025 - If you are looking for computational tools for your cancer research, join us for this upcoming training session on Galaxy, an open-source and browser-based computational platform. Dr. Jeremy Goecks, an NCI grantee and Assistant Read More...

06/25/2025 - Join tony Brook Univer ity&r quo; Dr. Joel altz a he di cu e : the role of pathology artificial intelligence (AI) in the creation of pathology imaging biomarker , including tumor infiltrating lymphocyte Read More...

05/01/2025 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering this two-part online training for non-statisticians interested in understanding the basic, intuitive thinking behind the Read More...

04/25/2025 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering this two-part online training for non-statisticians interested in understanding the basic, intuitive thinking behind the Read More...

04/10/2025 - This one hour online training introduces participants to the tools and techniques for analyzing and quantifying microscopy images using MATLAB’s low-code algorithms. Participants will learn how to preprocess images, segment regions Read More...

01/22/2025 - This three hour online training covers QIAGEN’s CLC Genomics Workbench enables researchers to analyze NGS data without the use of command line and is a powerful tool for determining differential expression. Read More...

01/21/2025 - About the Speaker: Hari completed his PhD in Cell Biology at the University of Toronto, where he used single-molecule imaging techniques to reveal how multimolecular interactions govern early signaling events in individual immune cells. Read More...

10/07/2024 - This one hour training, presented by speakers from  NIH Cloud Lab , will provide an overview of the generative artificial intelligence (AI) services across three major cloud service providers (CSPs), including Amazon Web Services (AWS), Read More...

08/13/2024 - NCI staff are welcome to register for an upcoming Childhood Cancer Data Initiative (CCDI) webinar titled, "CCDI Federated Data: Enhancing Data Discoverability," where you can learn about one of the newest advancements Read More...

07/11/2024 - Please join us for this special event featuring three speakers on the topic of Single-Cell Spatial Transcriptomics. George Zaki, Ph.D., Director, Applied Scientific Computing, BACS, The Frederick National Lab for Cancer Research, NCI/ Read More...

Single cell RNASeq is a remarkably powerful tool for analyzing populations of cells that can be recovered from various experiments. Clustering and cell type annotation can be used to distinguish different populations with a level Read More...

library(tidyverse) # dplyr and ggplot2; CRAN library(Seurat) # Seurat toolkit; CRAN library(hdf5r) # for data import; CRAN library(patchwork) # for plotting; CRAN library(presto) # for differential expression; Github library(glmGamPoi) # for sctransform; Bioconductor library( Read More...

This tutorial has been designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples that have already been filtered Read More...

04/30/2024 - What’s the difference between “regular” statistics (i.e., what you may have been using in the past several years) and the “new” Bayesian Read More...

04/23/2024 - What’s the difference between “regular” statistics (i.e., what you may have been using in the past several years) and the “new” Bayesian Read More...

The Advanced Biomedical Computational Science (ABCS) group focuses on applications of bioinformatics, computational and data science, and artificial intelligence to support NCI researchers. ABCS provides: • Subject matter expertise in genomics, proteomics, and imaging. • Machine learning/ Read More...

01/19/2024 - Trey Ideker, Ph.D., is a professor of medicine, bioengineering, and computer science, and former chief of genetics at the University of California San Diego (UCSD). Additionally, he is director or co-director of the Read More...

12/02/2026 - Partek Flow is a point-and-click platform for building analysis workflows for Next Generation Sequences (NGS), including DNA, bulk and single-cell RNA, spatial transcriptomics, ATAC, and ChIP, helping scientists avoid the steep learning curve of Read More...

08/19/2026 - Partek Flow is a point-and-click platform for building analysis workflows for Next Generation Sequences (NGS), including DNA, bulk and single-cell RNA, spatial transcriptomics, ATAC, and ChIP, helping scientists avoid the steep learning curve of Read More...

04/16/2026 - The ability to measure gene expression levels for individual cells (vs. pools of cells) and with spatial resolution is crucial to address many important biological and medical questions, such as the study of stem Read More...

02/18/2026 - Partek Flow is a point-and-click platform for building analysis workflows for Next Generation Sequences (NGS), including DNA, bulk and single-cell RNA, spatial transcriptomics, ATAC, and ChIP, helping scientists avoid the steep learning curve of Read More...

02/12/2026 - The NLM Division of Intramural Research (DIR) is pleased to welcome Tiarnán Keenan, MD, PhD, Stadtman Tenure-Track Investigator and Director of the Medical Retina Fellowship Program at the National Eye Institute Read More...

01/21/2026 - Alzheimer’s disease and related dementias (ADRD) remain a major health crisis with profound social and economic burdens. Innovative strategies are needed to identify genetic risk and protective factors, model disease mechanisms, Read More...

01/13/2026 - The past decade has witnessed thriving cell atlas research activities in elucidating human cell types and their organization principles. The NIH BRAIN Initiative launched a pilot 3-year BRAIN Initiative Cell Census Consortium (BICCC) in 2014, Read More...

12/10/2025 - Partek Flow enables scientists to construct analysis workflows for multi-omics sequencing data including DNA, bulk and single cell RNA, spatial transcriptomics, ATAC and ChIP. It is a point-and-click software suitable for those who wish Read More...

11/06/2025 - Qlucore Omics Explorer is a point-and-click software that enables analysis of RNA sequencing (bulk and single cell), proteomics and metabolomics data. It’s machine learning capabilities allow for cell type classification. This Read More...

10/30/2025 - Qiagen Ingenuity Pathway Analysis (IPA) is a point-and-click software that enables scientists to discern how genomic, transcriptomic, proteomic, and metabolomic changes influence molecular biology pathways and networks. This software is available to NCI investigators. Read More...

10/23/2025 - Qiagen CLC Genomics Workbench is a point-and-click software that runs on a personal computer and enables bulk RNA sequencing, ChIP sequencing, long reads, and variant analysis that is available to NCI scientists. Submit a Read More...

09/09/2025 - This event will be in-person only, with no Webex/hybrid option.  We will record the sessions and make them available in the BTEP Video Archive. There is no registration necessary.  Read More...

06/18/2025 - Gene et enrichment analy i (G EA) i a popular tool for evaluating functional enrichment, e pecially following differential expre ion analy i . Thi e ion of the BTEP Coding Club will how attendee Read More...

05/21/2025 - The programming language R i ideal for biomedical re earcher a it ha package that facilitate Next Generation equencing (NG ) data analy i . For example, bulk RNA equencing differential expre ion analy i can Read More...

04/08/2025 - Reactome is a free, open-source, curated and peer-reviewed pathway database that includes bioinformatics tools for the visualization, interpretation and analysis of pathway knowledge. This lesson will showcase the capabilities of Reactome in garnering biological Read More...

03/20/2025 - Please note: Registration is required to get the Meeting Link for this event. Please pre-register. BTEP and the Single Cell and Spatial Transcriptomics Interest Group jointly present: Quantifying spatiotemporal dynamics during embryogenesis is crucial Read More...

03/13/2025 - UCSC Xena showcases seminal cancer genomics datasets from The Cancer Genome Atlas (TCGA) and the Pan-Cancer Atlas, as well as the Genomic Data Commons, Pan-cancer Atlas of Whole Genomes, and the International Cancer Genome Read More...

12/12/2024 - This class will introduce bulk RNA sequencing analysis using Qiagen software. Participants will learn how to process FASTQ files and obtain differential expression using CLC Genomics Workbench as well as extract biological insight using Read More...

12/04/2024 - Galaxy is a web-based computational workbench for analyzing and visualizing biomedical datasets that is used by thousands of scientists daily.   Galaxy enables accessible, reproducible, and scalable biomedical data science by any scientist Read More...

What is bioinformatics? Bioinformatics integrates biology, statistics, and computer science to develop and apply theory, methods, and tools for the collection, storage, and analysis of biological and related data. Bioinformatics plays a critical role in Read More...

11/13/2024 - The National Library of Medicine (NLM) Division of Intramural Research (DIR) is pleased to welcome Manisha Desai, PhD, Section Chief of Biostatistics and Director of the Quantitative Sciences Unit at Stanford University School of Read More...

11/08/2024 - Learn microbiome analysis basics in R with phyloseq. This workshop will cover different types of analysis frequently used in microbiome studies, including sample diversity, community composition, and differential taxa. The techniques we learn will Read More...

10/09/2024 - This session will give an overview of the NCI Cancer Research Data Commons and the variety of available cancer data such as RNA expression and protein abundance. These data are hosted in openly accessible Read More...

06/13/2024 - The schedule for this week consists of one presentation: Lorenz Adlung, UMC Hamburg-Eppendorf, will discuss: scMod: Marrying machine learning and deterministic modelling of longitudinal single-cell data Single-cell-based methods such as flow cytometry or single-cell Read More...

library(tidyverse) # dplyr and ggplot2 library(Seurat) # Seurat toolkit library(hdf5r) # for data import library(patchwork) # for plotting library(presto) # for differential expression library(glmGamPoi) # for sctransform Warning: package 'glmGamPoi' was built under R Read More...

April 3, 2024 - The CCR Single Cell Analysis Facility (SCAF): An Overview (Mike Kelly, SCAF) ( Recording ) April 10, 2024 - Introduction to single cell RNA-Seq (Charlie Seibert, Saeed Yadranji Aghdam, SCAF) ( Recording ) April 17, 2024 - SCAF: Overview of Cell Read More...

Now, that we have clusters, we can use differential expression analysis to uncover markers that define our clusters. These markers can be used to assign cell types to our clusters. First, because we are working Read More...

Welcome to Getting Started with scRNA-Seq This is a mini seminar series designed to help attendees learn more about single cell RNA-Seq, from applicable technologies to data analysis. Seminar Schedule April 3, 2024 - The CCR Single Read More...

Many experiments look to compare to distinct populations, and scRNASeq is no exception. The two populations being compared can vary wildly from experiment to experiment; some look to draw comparisons based on the experimental condition, Read More...

05/23/2024 - An exciting opportunity at the intersection of the biomedical sciences and machine learning stems from the growing availability of large-scale multi-modal data (imaging-based and sequencing-based, observational and perturbational, at the single-cell level, tissue-level, and Read More...

05/08/2024 - Qlucore Omics Explorer is a point-and-click software that enables analysis of RNA sequencing (bulk and single cell), proteomics and metabolomics data. It’s machine learning capabilities also allow for classification of cell types. This Read More...

04/24/2024 - Please join us on Wednesday, April 24, 2024, when Dr. Abhishek Jha, co-founder and CEO of Elucidata, will present " Data Quality for LLMs: Building a Reliable Data Foundation." The presentation starts at 11:00 a.m. Read More...

04/01/2024 - Embark on a journey of visionary insights! Join us for the launch of the NEI Informatics & Data-Driven Insights: Seminars & Dialogue Opportunities for Vision Health series, hosted by the National Eye Read More...

03/29/2024 - This event is sponsored by an organization outside of the NIH; it is listed here due to the nature of the presented topics and their appeal to the NCI community. Description: Recent developments in Read More...

03/20/2024 - Our series of talks continues with two 20-minute presentations.  There will be light refreshments (coffee and donuts) provided, so please consider attending in person! We encourage attendees to stay and chat with Read More...

03/13/2024 - Dear Colleagues,    UCSC Xena is a web-based visual integration and exploration tool for multi-omic data and associated clinical and phenotypic annotations.   Xena showcases seminal cancer genomics datasets from Read More...

Learning Objectives This tutorial was designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples. Throughout this tutorial we Read More...

Most BTEP courses include detailed course materials including lesson content, additional resources, and lesson associated data. These course materials are listed here so that learners can easily return to and review concepts taught in class Read More...

  Update: Please contact your IC representative to get access to the Anaconda Business license as it has transitioned from enterprise to seat-based licensing. For questions, email anaconda@nih.gov. Anaconda ( https://www.anaconda. Read More...

The computational chemistry and protein modeling team in the Advanced Biomedical Computational Science (ABCS) group provides novel solutions in structural modeling and computational chemistry. Computational scientists in the group collaborate with NCI researchers by using Read More...

Listed below are the video recordings of past BTEP events (classes, seminars, workshops). Videos are hosted on various servers and may play slightly differently. Some videos may be downloaded for local viewing. Recorded Videos of Read More...

12/05/2024 - This one and a half hour online training will provide a demonstration of how to identify cell types based on statistics, visualization, and canonical markers. One Peripheral blood mononuclear cells (PBMCs) sample will be Read More...

11/19/2024 - Partek Flow enables scientists to construct analysis workflows for multi-omics sequencing data including DNA, bulk and single cell RNA, spatial transcriptomics, ATAC and ChIP. It is a point-and-click software hosted on Biowulf, the NIH Read More...

Congratulations, your experiments have been completed and you have a large amount of data. Now, it’s time to analyze these data. But where do you begin? How can you gain the most meaning from Read More...

06/12/2024 - Join us for an engaging training session where we will examine the similarities and differences between machine learning and statistical differential gene expression (DGE) analysis using Qlucore Omics Explorer. Qlucore Omics Explorer is a Read More...

As we can see above, the glimpse command shows the metadata that can be used to classify the cells. Within Seurat, the metadata is used to define the "identity" of the dataset. This Read More...

Much like pseudobulk differential expression, the RNA expression can be collapsed into pre-defined components, such as the clusters, if it is believed that cell-to-cell variation is inducing too much confusion in the labeling. This collapsing Read More...

Here, we will start with the data stored in a Seurat object. For instructions on data import and creating the object, see an Introduction to scRNA-Seq with R (Seurat) and Getting Started with Seurat: QC Read More...

The FindAllMarkers function is particularly useful in identifying the differentially expressed genes that distinguish several groups, such as seen here in the clusters. What makes this unique is that none of the identities are initially Read More...

This lesson provides an introduction to R in the context of single cell RNA-Seq analysis with Seurat. Learning Objectives Learn about options for analyzing your scRNA-Seq data. Learn about resources for learning R programming. Learn Read More...

05/16/2024 - Qiagen CLC Genomics Workbench is a point-and-click bioinformatics software that runs on a personal computer and enables bulk RNA sequencing, ChIP sequencing, long reads, and variant analysis. NCI scientists can use CLC Genomics Workbench Read More...